Lafarga M, Berciano M T, Hervas J P, Villegas J
Department of Anatomy and Cell Biology, University of Cantabria, Faculty of Medicine, Santander, Spain.
J Neurocytol. 1989 Feb;18(1):19-26. doi: 10.1007/BF01188420.
The present light and electron microscopic study deals with the morphology and cytochemical properties of the nucleolus in mature granule cells of the rat cerebellum. Nucleolar organization has been examined by several different methodological approaches: (a) determination of the number of nucleoli per cell from smear preparations, (b) morphometric estimation of nucleolar size, and (c) ultrastructural and cytochemical characterization of nucleolar components. The mean number of nucleoli per cell was found to be 1.46 +/- 0.02 (mean +/- SEM). The morphometric measurement of the nucleolar area showed an average value of 0.359 +/- 0.07 microns 2 (mean +/- SDM). At the electron microscope level, most granule cell nucleoli displayed a fine texture which does not differ substantially from the 'ring-shaped' configuration usually found in cells with low levels of protein synthesis activity; i.e., a large round fibrillar centre surrounded by the dense fibrillar component, and small masses of granular component segregated from the preceding constituents. The meaning of this nucleolar configuration is discussed in the light of recent ultrastructural and biochemical data on nucleolar function. A relationship between this pattern of nucleolar organization and metabolic characteristics of the granule cells is suggested.
本项光镜和电镜研究涉及大鼠小脑成熟颗粒细胞中核仁的形态学和细胞化学特性。已通过几种不同的方法对核仁组织进行了研究：(a) 从涂片制备物中确定每个细胞的核仁数量，(b) 对核仁大小进行形态计量估计，以及 (c) 对核仁成分进行超微结构和细胞化学表征。发现每个细胞的核仁平均数为1.46±0.02（平均值±标准误）。核仁面积的形态计量测量显示平均值为0.359±0.07微米²（平均值±标准差）。在电子显微镜水平上，大多数颗粒细胞核仁呈现出精细的结构，与通常在蛋白质合成活性水平较低的细胞中发现的“环形”结构没有实质性差异；即，一个大的圆形纤维中心被致密纤维成分包围，以及与前两种成分分离的小颗粒成分团块。根据最近关于核仁功能的超微结构和生化数据讨论了这种核仁结构的意义。提出了这种核仁组织模式与颗粒细胞代谢特征之间的关系。
