Coetzee J, van der Merwe C F
Department of Electron Microscopy, University of Pretoria, South Africa.
J Electron Microsc Tech. 1989 Feb;11(2):155-60. doi: 10.1002/jemt.1060110210.
Loss of 14C-labeled compounds from bean leaf tissue was monitored during all the stages of routine specimen preparation. No significant differences in extraction were associated with the use of acetone, ethanol, or dioxane as dehydration fluids. Fixation at low temperature increased the loss of label. Prolonged fixation in glutaraldehyde increased the loss, but fixation in osmium solutions for periods as long as 4 hr had no influence on extraction. Buffer rinses and dehydration fluids caused appreciable amounts of label to be extracted. The use of propylene oxide as transition fluid resulted in low extraction. Some embedding media caused the loss of small amounts of labeled compounds, but one of the media tested (LR-white) extracted significant amounts of label.
在常规标本制备的所有阶段，监测了蚕豆叶片组织中¹⁴C标记化合物的损失情况。使用丙酮、乙醇或二氧六环作为脱水剂时，提取物中没有显著差异。低温固定会增加标记物的损失。在戊二醛中长时间固定会增加损失，但在锇溶液中固定长达4小时对提取物没有影响。缓冲液冲洗和脱水剂会导致大量标记物被提取。使用环氧丙烷作为过渡液会导致提取物量较低。一些包埋介质会导致少量标记化合物的损失，但测试的一种介质（LR-白色）提取了大量标记物。
