Sakamoto Seiichi, Kohno Toshitaka, Shimizu Kuniyoshi, Tanaka Hiroyuki, Morimoto Satoshi
Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, Higashi-ku, Fukuoka, Japan.
Department of Agro-Environmental Sciences, Graduate School of Agriculture, Kyushu University, Higashi-ku, Fukuoka, Japan.
Planta Med. 2016 May;82(8):747-51. doi: 10.1055/s-0042-104202. Epub 2016 Apr 19.
Ganoderma is a genus of medicinal mushroom traditionally used for treating various diseases. Ganoderic acid A is one of the major bioactive Ganoderma triterpenoids isolated from Ganoderma species. Herein, we produced a highly specific monoclonal antibody against ganoderic acid A (MAb 12 A) and developed an indirect competitive ELISA for the highly sensitive detection of ganoderic acid A in Ganoderma lingzhi, with a limit of detection of 6.10 ng/mL. Several validation analyses support the accuracy and reliability of the developed indirect competitive ELISA for use in the quality control of Ganoderma based on ganoderic acid A content. Furthermore, quantitative analysis of ganoderic acid A in G. lingzhi revealed that the pileus exhibits the highest ganoderic acid A content compared with the stipe and spore of the fruiting body; the best extraction efficiency was found when 50 % ethanol was used, which suggests the use of a strong liquor to completely harness the potential of Ganoderma triterpenoids in daily life.
灵芝是一类传统上用于治疗各种疾病的药用真菌。灵芝酸A是从灵芝属物种中分离出的主要生物活性灵芝三萜类化合物之一。在此，我们制备了一种针对灵芝酸A的高特异性单克隆抗体（单克隆抗体12 A），并开发了一种间接竞争ELISA法，用于高灵敏度检测灵芝中的灵芝酸A，检测限为6.10 ng/mL。多项验证分析支持所开发的间接竞争ELISA法基于灵芝酸A含量用于灵芝质量控制的准确性和可靠性。此外，对灵芝中灵芝酸A的定量分析表明，与子实体的菌柄和孢子相比，菌盖的灵芝酸A含量最高；使用50%乙醇时提取效率最佳，这表明在日常生活中使用高度酒可以充分发挥灵芝三萜类化合物的潜力。
