植物一氧化氮（NO）研究方法概述：为何我们总是需要使用多种方法？

An Overview of Methods in Plant Nitric Oxide (NO) Research: Why Do We Always Need to Use Multiple Methods?

作者信息

Yamasaki Hideo, Watanabe Naoko S, Sakihama Yasuko, Cohen Michael F

机构信息

Faculty of Science, University of the Ryukyus, Nishihara, Okinawa, 903-0213, Japan.

Research Faculty of Agriculture, Hokkaido University, Kita Ku, Kita 9, Nishi 9, Sapporo, Hokkaido, 060-8589, Japan.

出版信息

Methods Mol Biol. 2016;1424:1-14. doi: 10.1007/978-1-4939-3600-7_1.

DOI:10.1007/978-1-4939-3600-7_1

PMID:27094406

Abstract

The free radical nitric oxide (NO) is a universal signaling molecule among living organisms. To investigate versatile functions of NO in plants it is essential to analyze biologically produced NO with an appropriate method. Owing to the uniqueness of NO, plant researchers may encounter difficulties in applying methods that have been developed for mammalian study. Based on our experience, we present here a practical guide to NO measurement fitted to plant biology.

摘要

自由基一氧化氮（NO）是生物体内一种普遍存在的信号分子。为了研究NO在植物中的多种功能，采用合适的方法分析生物体内产生的NO至关重要。由于NO的独特性，植物研究人员在应用已开发用于哺乳动物研究的方法时可能会遇到困难。基于我们的经验，在此提供一份适用于植物生物学的NO测量实用指南。