Ren Xianyun, Cui Yanting, Gao Baoquan, Liu Ping, Li Jian
Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, PR China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, PR China.
Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, PR China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, PR China.
Mar Genomics. 2016 Aug;28:113-120. doi: 10.1016/j.margen.2016.03.010. Epub 2016 Apr 16.
MicroRNAs (miRNAs) are a class of endogenous small non-coding RNAs that regulate gene expression by post-transcriptional repression of mRNAs. The swimming crab Portunus trituberculatus is one of the most important crustacean species for aquaculture in China. However, to date no miRNAs have been reported to for modulating growth in P. trituberculatus. To investigate miRNAs involved in the growth of this species, we constructed six small RNA libraries for big individuals (BIs) and small individuals (SIs) from a highly inbred family. Six mixed RNA pools of five tissues (eyestalk, gill, heart, hepatopancreas, and muscle) were obtained. By aligning sequencing data with those for known miRNAs, a total of 404 miRNAs, including 339 known and 65 novel miRNAs, were identified from the six libraries. MiR-100 and miR-276a-3p were among the most prominent miRNA species. We identified seven differentially expressed miRNAs between the BIs and SIs, which were validated using real-time PCR. Preliminary analyzes of their putative target genes and GO and KEGG pathway analyzes showed that these differentially expressed miRNAs could play important roles in global transcriptional depression and cell differentiation of P. trituberculatus. This study reveals the first miRNA profile related to the body growth of P. trituberculatus, which would be particularly useful for crab breeding programs.
微小RNA（miRNA）是一类内源性小非编码RNA，通过对mRNA进行转录后抑制来调节基因表达。三疣梭子蟹是中国水产养殖中最重要的甲壳类物种之一。然而，迄今为止，尚未有关于三疣梭子蟹中调节生长的miRNA的报道。为了研究参与该物种生长的miRNA，我们从一个高度近交的家系中构建了大个体（BIs）和小个体（SIs）的六个小RNA文库。获得了五个组织（眼柄、鳃、心脏、肝胰腺和肌肉）的六个混合RNA池。通过将测序数据与已知miRNA的数据进行比对，从这六个文库中总共鉴定出404个miRNA，包括339个已知miRNA和65个新miRNA。MiR-100和miR-276a-3p是最突出的miRNA种类。我们在BIs和SIs之间鉴定出七个差异表达的miRNA，并使用实时PCR进行了验证。对其假定靶基因的初步分析以及GO和KEGG通路分析表明，这些差异表达的miRNA可能在三疣梭子蟹的全局转录抑制和细胞分化中发挥重要作用。本研究揭示了首个与三疣梭子蟹身体生长相关的miRNA图谱，这对蟹类育种计划将特别有用。
