Glińska-Suchocka K, Sławuta P, Jankowski M, Kubiak K, Spużak J, Borusewicz P
Pol J Vet Sci. 2016;19(1):141-5. doi: 10.1515/pjvs-2016-0018.
The aim of the study was to assess pH, pO2 and pCO2 in peritoneal fluid. The study was conducted on a group of 22 dogs with symptoms of ascites. Group 1 consisted of 4 dogs with adenocarcinoma, group 2--of 6 dogs with glomerulonephritis, group 3 of 8 dogs with hepatic cirrhosis and group 4 of 4 dogs with bacterial peritonitis. An abdominal cavity puncture was performed in all dogs and the fluid was drawn into a heparinized syringe in order to assess pH, pO2 and pCO2 . The analysis of pH in the peritoneal fluid revealed statistically significant differences between group 4 and groups 1 (p=0.01), 2 (p=0.01), and 3 (p=0.01). The lowest pH value compared to the other studied groups was recorded in group 4. In group 4, the pO2 was the lowest compared to the other groups (group 1 p=0.01, group 2 p=0.01, group 3 p=0.01). The value of pCO2 was the highest in group 4 compared to groups 1, 2, and 3. The study found statistically significant differences in pH, pCO2 and pCO2 between group 4 (the group of dogs with bacterial peritonitis) and the other groups of dogs. This was probably linked to the pathogenesis of peritonitis. As a result of an inflammatory reaction within the peritoneal cavity, there is an increase in fibrin accumulations leading to a decreased oxygen supply causing the oxidative glucose metabolism to change into a non-oxidative glucose metabolism. This, in turn, causes a decrease in pH, acidosis, and a low oxidoreduction potential. It also impairs phagocytosis and activates proteolytic enzymes which create ideal conditions for the growth of anaerobic bacteria. The obtained results indicate that the pH, pO2 and pCO2 may be used to differentiate bacterial peritonitis from ascites of other etiologies.
该研究的目的是评估腹腔液中的pH值、氧分压（pO2）和二氧化碳分压（pCO2）。研究针对一组22只有腹水症状的犬进行。第1组由4只患有腺癌的犬组成，第2组由6只患有肾小球肾炎的犬组成，第3组由8只患有肝硬化的犬组成，第4组由4只患有细菌性腹膜炎的犬组成。对所有犬进行腹腔穿刺，并将液体吸入肝素化注射器中，以评估pH值、pO2和pCO2。腹腔液pH值分析显示，第4组与第1组（p = 0.01）、第2组（p = 0.01）和第3组（p = 0.01）之间存在统计学上的显著差异。与其他研究组相比，第4组记录到最低的pH值。在第4组中，pO2与其他组相比是最低的（第1组p = 0.01，第2组p = 0.01，第3组p = 0.01）。与第1、2和3组相比，第4组的pCO2值最高。该研究发现，第4组（患有细菌性腹膜炎的犬组）与其他犬组之间在pH值、pCO2和pO2方面存在统计学上的显著差异。这可能与腹膜炎的发病机制有关。由于腹腔内的炎症反应，纤维蛋白积聚增加，导致氧气供应减少，使氧化葡萄糖代谢转变为非氧化葡萄糖代谢。这进而导致pH值降低、酸中毒和低氧化还原电位。它还会损害吞噬作用并激活蛋白水解酶，为厌氧菌的生长创造理想条件。获得的结果表明，pH值、pO2和pCO2可用于区分细菌性腹膜炎与其他病因引起的腹水。
