Ryu Seung-Tae, Park Byung-Sung, Bang Han-Tae, Kang Hwan-Ku, Hwangbo Jong
J Environ Biol. 2016 Mar;37(2):185-92.
The present study investigated the effects of night restricted feeding of extreme heat diet (EHD) containing heat stress resistance nutrients, with inverse lighting program, on their growth performance in broiler chickens exposed to extreme heat stress (33 ± 2°C). EHD 1 contained soy oil, molasses, methionine and lysine. EHD 2 contained all nutrients of EHD 1 and vitamin C additionally. Three hundred broiler chickens (Ross 308) were randomized into four dietary treatment groups according to a randomized block design on the day they were hatched. The treatment groups were: T1 (EHD 1, 10:00-19:00 dark, 19:00-10:00 light), T2 (EHD 2, 10:00-19:00 dark, 19:00-10:00 light), T3 (EHD 1, 09:00-18:00 dark, 18:00-09:00 light) and T4 (EHD 2, 09:00-18:00 dark, 18:00-09:00 light). The body weight gain of the broilers increased most significantly in T2, followed by T1, T4 and T3 (p < 0.05). Weights of the immune system, thymus and bursa of Fabricius recorded higher in T1 and T2 than in T3 and T4. The spleen was higher in T1, T2 and T3 than in T4 (p < 0.05). Blood triglyceride, total cholesterol and blood sugar were higher in T1 and T2 than in T3 and T4 (p < 0.05). LDL-C recorded high in the order of T4, T3, T2 and T1, but HDL-C showed the inverse order (p < 0.05). IgG, IgA and IgM were higher in T1 and T2 than in T3 and T4, however, the corticosterone concentration showed the inverse order (p<0.05). Lactobacillus in feces was higher in T1 and T2 than in T3 and T4, but total aerobic bacteria, E. coli, Coliform bacteria was higher in T4 and T3 than in T2 and T1 (p < 0.05). Contents of acetic acid, propionic acid and total Short chain fatty acid were significantly higher in the order of T2, T1, T3 and T4. Butyric acid, isobutyric acid, valeric acid and isovaleric acid were higher in T4 and T3 than in T1 and T2 (p < 0.05).
本研究调查了含抗热应激营养成分的极热日粮（EHD）夜间限时投喂，并采用反向光照程序，对暴露于极热应激（33±2°C）下的肉鸡生长性能的影响。EHD 1包含大豆油、糖蜜、蛋氨酸和赖氨酸。EHD 2除了包含EHD 1的所有营养成分外，还额外添加了维生素C。300只肉鸡（罗斯308）在孵化当天根据随机区组设计随机分为四个日粮处理组。处理组分别为：T1（EHD 1，10:00 - 19:00黑暗，19:00 - 10:00光照），T2（EHD 2，10:00 - 19:00黑暗，19:00 - 10:00光照），T3（EHD 1，09:00 - 18:00黑暗，18:00 - 09:00光照）和T4（EHD 2，09:00 - 18:00黑暗，18:00 - 09:00光照）。肉鸡的体重增加在T2组最为显著，其次是T1组、T4组和T3组（p<0.05）。T1组和T2组的免疫系统、胸腺和法氏囊重量高于T3组和T4组。T1组、T2组和T3组的脾脏重量高于T4组（p<0.05）。T1组和T2组的血液甘油三酯、总胆固醇和血糖高于T3组和T4组（p<0.05）。低密度脂蛋白胆固醇（LDL-C）按T4、T3、T2、T1的顺序升高，但高密度脂蛋白胆固醇（HDL-C）呈相反顺序（p<0.05）。T1组和T2组的免疫球蛋白G（IgG）、免疫球蛋白A（IgA）和免疫球蛋白M（IgM）高于T3组和T4组，然而，皮质酮浓度呈相反顺序（p<0.05）。T1组和T2组粪便中的乳酸杆菌高于T3组和T4组，但总需氧菌、大肠杆菌、大肠菌群在T4组和T3组高于T2组和T1组（p<0.05）。乙酸、丙酸和总短链脂肪酸含量按T2、T1、T3、T4的顺序显著升高。丁酸、异丁酸、戊酸和异戊酸在T4组和T3组高于T1组和T2组（p<0.05）。
