Saxena Ankita, Kesari V P
J Environ Biol. 2016 Mar;37(2):291-5.
Pesticides, spinosad, imidacloprid and neem oil are widely used both in residential and agricultural environments because of its broad spectrum insecticidal activity and effectiveness. The present study was undertaken to estimate genotoxicity of formulations of some pesticides in mice. Three pesticides of diverse group studied were spinosad (45% w/v), imidacloprid (17.8%, w/v) and neem oil. Animals were exposed 37, 4.5 and 50 mg kg⁻¹ b.wt. for spinosad, imidacloprid and neem oil, respectively, through oral gavage for 5 consecutive days. A vehicle control group and one positive control (cyclophosphamide; 20 mg kg⁻¹ b. wt.) were also selected. The results showed that cyclophosphamide produced 1.12% micronuclei in mice, as against 0.18 in vehicle control, 0.30 in spinosad, 0.28 in imidacloprid and 0.22% in neem oil, respectively. The gross percentage of chromosomal aberration in mice were 28.5% in cyclophosphamide against 6.5% in vehicle control, 8.0% in spinosad, 9.5% in imidacloprid and 7.0% in neem oil, respectively. The overall findings of the present study revealed that all the three pesticide formulations, imidacloprid, spinosad and neem oil at tested dose did not show any genotoxic effect in mice.
由于具有广谱杀虫活性和有效性，杀虫剂多杀菌素、吡虫啉和印楝油在住宅和农业环境中都被广泛使用。本研究旨在评估某些杀虫剂制剂对小鼠的遗传毒性。所研究的三种不同类型的杀虫剂分别是多杀菌素（45% w/v）、吡虫啉（17.8%，w/v）和印楝油。分别以37、4.5和50 mg kg⁻¹体重的剂量，通过口服灌胃连续5天让动物接触多杀菌素、吡虫啉和印楝油。还选择了一个溶剂对照组和一个阳性对照组（环磷酰胺；20 mg kg⁻¹体重）。结果显示，环磷酰胺使小鼠产生1.12%的微核，而溶剂对照组为0.18%，多杀菌素组为0.30%，吡虫啉组为0.28%，印楝油组为0.22%。小鼠染色体畸变的总百分比在环磷酰胺组为28.5%，溶剂对照组为6.5%，多杀菌素组为8.0%，吡虫啉组为9.5%，印楝油组为7.0%。本研究的总体结果表明，在所测试剂量下，吡虫啉、多杀菌素和印楝油这三种农药制剂在小鼠中均未显示出任何遗传毒性作用。
