Hao Feiran, Fu Bin, Zhang Yangjun, Qian Xiaohong
Se Pu. 2015 Dec;33(12):1226-33. doi: 10.3724/sp.j.1123.2015.09010.
Preparative electromigration techniques are a category of separation and preparation techniques based on differential electromigration principle. These techniques are widely applied in biomacromolecule and proteome researches. Related techniques mainly include preparative electrophoresis, preparative electrochromatography, preparative isoelectric focusing and free flow electrophoresis and so on. We review each kind of the preparative electromigration techniques on its apparatus designs, applications, advantages and disadvantages in detail. Microscale preparative electrophoresis is a hot spot in recent years. Its high resolution, high recovery and high efficiency make it a more prominent role in the biological sample analysis. In this review, we focus on some advances in microscale preparative electrophoresis and also give an outlook on preparative electromigration techniques in the future.
制备性电迁移技术是一类基于微分电迁移原理的分离和制备技术。这些技术在生物大分子和蛋白质组研究中得到广泛应用。相关技术主要包括制备性电泳、制备性电色谱、制备性等电聚焦和自由流动电泳等。我们详细综述了每种制备性电迁移技术的仪器设计、应用、优缺点。微尺度制备性电泳是近年来的一个热点。其高分辨率、高回收率和高效率使其在生物样品分析中发挥更突出的作用。在本综述中，我们重点关注微尺度制备性电泳的一些进展，并对制备性电迁移技术的未来进行展望。
