Ji Xiao-Yi, Wu Min
Department of Pediatrics, Jiaxing Maternity and Child Health Care Hospital, Jiaxing, Zhejiang 314000, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2016 Apr;18(4):297-300. doi: 10.7499/j.issn.1008-8830.2016.04.003.
To investigate the mRNA expression of dopamine receptor D2 (DRD2) and dopamine transporter (DAT) in peripheral blood lymphocytes before and after treatment in children with tic disorder (TD).
RT-PCR was used to measure the mRNA expression of DRD2 and DAT in peripheral blood lymphocytes before and after treatment in 60 children with TD. The correlations between mRNA expression of DRD2 and DAT and the severity of TD were analyzed. Sixty healthy children served as the control group.
Before treatment, the children with TD had a significant increase in the mRNA expression of DRD2 and DAT compared with the control group (P<0.05). After 3 months of treatment with oral aripiprazole, the mRNA expression of DRD2 decreased significantly (P<0.05), while that of DAT showed no significant changes in children with TD. In the children with moderate or severe TD, the mRNA expression of DRD2 was positively correlated with Yale Global Tic Severity Scale (YGTSS) score (P<0.05). In the children with moderate TD, the mRNA expression of DAT was positively correlated with YGTSS score (P<0.05).
In children with TD, the mRNA expression of DRD2 in peripheral blood lymphocytes can be used as one of the indicators for diagnosing TD, assessing the severity of TD, and evaluating clinical outcomes.
探讨抽动障碍（TD）患儿治疗前后外周血淋巴细胞中多巴胺受体D2（DRD2）和多巴胺转运体（DAT）的mRNA表达情况。
采用逆转录-聚合酶链反应（RT-PCR）检测60例TD患儿治疗前后外周血淋巴细胞中DRD2和DAT的mRNA表达。分析DRD2和DAT的mRNA表达与TD严重程度之间的相关性。60例健康儿童作为对照组。
治疗前，TD患儿DRD2和DAT的mRNA表达较对照组显著升高（P<0.05）。口服阿立哌唑治疗3个月后，TD患儿DRD2的mRNA表达显著降低（P<0.05），而DAT的mRNA表达无明显变化。在中度或重度TD患儿中，DRD2的mRNA表达与耶鲁综合抽动严重程度量表（YGTSS）评分呈正相关（P<0.05）。在中度TD患儿中，DAT的mRNA表达与YGTSS评分呈正相关（P<0.05）。
在TD患儿中，外周血淋巴细胞中DRD2的mRNA表达可作为诊断TD、评估TD严重程度及评价临床疗效的指标之一。
