Shen Y-H, Xie Z-B, Yue A-M, Wei Q-D, Zhao H-F, Yin H-D, Mai W, Zhong X-G, Huang S-R
Department of Oncological Surgery, Xinxiang Central Hospital, Xinxiang, Henan, P.R. China.
Eur Rev Med Pharmacol Sci. 2016 Apr;20(7):1283-7.
To study miroRNA-195 (miR-195) expression in the serum and cancer tissue of patients with gastric cancer and to investigate the relationship between its expression and clinicopathological features of gastric cancer.
Sixty-two patients with gastric cancer admitted to our institution were included in the study group, and 36 healthy persons undergoing health check-up at our institution served as control group. miR-195 expressions in the serum, gastric cancer tissue and corresponding paracancerous tissue in subjects of two groups were measured by using quantitative fluorescent real-time PCR (QF-RT-PCR), and the relationship between miR-195 and the clinicopathological features of the cancer was investigated.
miR-195 expression level in the serum of gastric cancer patients was significantly lower than that in the control group (p <0.05). miR-195 expression in gastric cancer tissue was also significantly lower than that in corresponding paracancerous tissue (p <0.05). The results of correlation analysis showed that low expression of miR-195 was negatively correlated with the infiltration depth, the extent of differentiation, the clinical staging and lymph node metastasis, all with statistical significance (p <0.05), but not significantly correlated with tumor locations (p >0.05).
Low expression of miR-195 in patients with gastric cancer may play a certain role in promoting the genesis and development of gastric cancer and it can function as a potential novel tumor marker for the early diagnosis and prognosis evaluation of gastric cancer.
研究微小RNA-195（miR-195）在胃癌患者血清及癌组织中的表达情况，并探讨其表达与胃癌临床病理特征之间的关系。
选取我院收治的62例胃癌患者作为研究组，另选取在我院进行健康体检的36例健康人作为对照组。采用定量荧光实时聚合酶链反应（QF-RT-PCR）检测两组受试者血清、胃癌组织及相应癌旁组织中miR-195的表达情况，并分析miR-195与胃癌临床病理特征的关系。
胃癌患者血清中miR-195表达水平显著低于对照组（p<0.05）。胃癌组织中miR-195表达也显著低于相应癌旁组织（p<0.05）。相关性分析结果显示，miR-195低表达与浸润深度、分化程度、临床分期及淋巴结转移均呈负相关，差异均有统计学意义（p<0.05），但与肿瘤部位无明显相关性（p>0.05）。
胃癌患者miR-195低表达可能在胃癌的发生发展中起一定作用，可作为胃癌早期诊断及预后评估的潜在新型肿瘤标志物。
