一种用于在紧急毒理学环境中鉴定碱性和中性药物的多变量薄层及反相薄层色谱方案的评估。

Evaluation of a multiple-variable thin-layer and reversed-phase thin-layer chromatographic scheme for identification of basic and neutral drugs in an emergency toxicology setting.

作者信息

Harper J D, Martel P A, O'Donnell C M

机构信息

Toxi-Lab, Inc., Irvine, California 92718.

出版信息

J Anal Toxicol. 1989 Jan-Feb;13(1):31-6. doi: 10.1093/jat/13.1.31.

DOI:10.1093/jat/13.1.31

PMID:2709825

Abstract

An analytical scheme composed of one normal-phase thin-layer chromatographic (TLC) method, one reversed-phase thin-layer chromatographic (RPTLC) method, and sequential analyte detection through four stages of color reactions is described. Eighty-one basic or neutral drugs were analyzed with this scheme and seventy-four were uniquely characterized with 95% confidence. Six of the remaining seven formed three unresolved pairs. The scheme was evaluated by mean list length analysis and shown to offer analyte resolution similar to that of a scheme of TLC and gas chromatography (GC) with nonspecific detection. Nine out of ten unknowns from the field of eighty-one drugs were uniquely identified with 95% confidence by the TLC/RPTLC scheme. The tenth unknown was not completely resolved from its isomer, but was statistically the more probable candidate.

摘要

本文描述了一种分析方案，该方案由一种正相薄层色谱（TLC）方法、一种反相薄层色谱（RPTLC）方法以及通过四个显色反应阶段进行连续分析物检测组成。使用该方案对81种碱性或中性药物进行了分析，其中74种药物具有95%置信度的独特特征。其余7种药物中有6种形成了3对无法分离的组合。通过平均列表长度分析对该方案进行了评估，结果表明该方案提供的分析物分辨率与采用非特异性检测的TLC和气相色谱（GC）方案相似。在81种药物领域中，通过TLC/RPTLC方案对十分之九的未知物进行了95%置信度的唯一鉴定。第十种未知物与其异构体未完全分离，但在统计学上是更有可能的候选物。