一种用于嗅球点燃的非立体定位方法揭示了近交系小鼠品系之间不同的点燃率。

A non-stereotaxic method for olfactory bulb kindling reveals distinct kindling rates among inbred mouse strains.

作者信息

Green R C, Elizondo B J

机构信息

Department of Neurology, Children's Hospital, Boston, MA.

出版信息

J Neurosci Methods. 1989 Mar;27(2):109-13. doi: 10.1016/0165-0270(89)90093-9.

DOI:10.1016/0165-0270(89)90093-9

PMID:2709879

Abstract

A simple method for olfactory bulb kindling in the unrestrained mouse is described. Twisted wire electrodes attached to a dip-socket head assembly were implanted in the olfactory bulb. A ground lead was secured to a single skull screw and implantation was visually guided into the olfactory bulb. In all properly implanted preparations, stimulation produced an afterdischarge with behavioral responses which progressed to fully kindled convulsions. Location within the olfactory bulb did not affect the kindling rate. With this technique, mice from the C57BL/6J, C3H/He and DBA/2 inbred strains were kindled. Within each strain, kindling parameters were closely distributed about the group mean. Kindling rates in C3H and DBA inbred strains were significantly different from each other, but not significantly different from those reported in amygdala kindling.

摘要

本文描述了一种在无束缚小鼠中进行嗅球点燃的简单方法。将连接到双插座头部组件的双绞线电极植入嗅球。接地引线固定在单个颅骨螺钉上，并在视觉引导下将其植入嗅球。在所有正确植入的实验准备中，刺激会产生伴有行为反应的后放电，这种反应会发展为完全点燃惊厥。在嗅球内的位置并不影响点燃率。使用该技术，对C57BL/6J、C3H/He和DBA/2近交系小鼠进行了点燃实验。在每个品系中，点燃参数紧密分布在组均值周围。C3H和DBA近交系的点燃率彼此显著不同，但与杏仁核点燃实验中报道的点燃率无显著差异。