Birthare Karamveer, Shojaee Mozhgan, Jones Carlos Gross, Brenner James R, Bashur Chris A
Department of Biomedical Engineering, Florida Institute of Technology, 150 West University Blvd., Melbourne, FL 32901, USA.
Biomed Mater. 2016 Apr 21;11(2):025019. doi: 10.1088/1748-6041/11/2/025019.
Modulating the host response, including the accumulation of oxidized lipid species, is important for improving tissue engineered vascular graft (TEVG) viability. Accumulation of oxidized lipids promotes smooth muscle cell (SMC) hyper-proliferation and inhibits endothelial cell migration, which can lead to several of the current challenges for small-diameter TEVGs. Generating biomaterials that reduce lipid oxidation is important for graft survival and this assessment can provide a reliable correlation to clinical situations. In this study, we determined the collagen to poly(ε-caprolactone) (PCL) ratio required to limit the production of pro-inflammatory species, while maintaining the required mechanical strength for the graft. Electrospun conduits were prepared from 0%, 10%, and 25% blends of collagen/PCL (w/w) and implanted in the rat peritoneal cavity for four weeks. The results showed that adding collagen to the PCL conduits reduced the accumulation of oxidized lipid species within the implanted conduits. In addition, the ratio of collagen had a significant impact on the recruited cell phenotype and construct mechanics. All conduits exhibited greater than 44% yield strain and sufficient tensile strength post-implantation. In conclusion, these results demonstrate that incorporating collagen into synthetic electrospun scaffolds, both 10% and 25% blend conditions, appears to limit the pro-inflammatory characteristics after in vivo implantation.
调节宿主反应，包括氧化脂质物质的积累，对于提高组织工程血管移植物（TEVG）的存活率至关重要。氧化脂质的积累促进平滑肌细胞（SMC）过度增殖并抑制内皮细胞迁移，这可能导致小直径TEVG目前面临的一些挑战。生成减少脂质氧化的生物材料对于移植物存活很重要，并且这种评估可以提供与临床情况的可靠关联。在本研究中，我们确定了限制促炎物质产生所需的胶原蛋白与聚（ε-己内酯）（PCL）的比例，同时保持移植物所需的机械强度。由0%、10%和25%（重量/重量）的胶原蛋白/PCL混合物制备电纺导管，并植入大鼠腹腔四周。结果表明，向PCL导管中添加胶原蛋白可减少植入导管内氧化脂质物质的积累。此外，胶原蛋白的比例对募集细胞的表型和构建体力学有显著影响。所有导管在植入后均表现出大于44%的屈服应变和足够的拉伸强度。总之，这些结果表明，在10%和25%混合条件下，将胶原蛋白掺入合成电纺支架中似乎可以限制体内植入后的促炎特性。
