Nikhil Vineeta, Jaiswal Shikha, Bansal Parul, Arora Rohit, Raj Shalya, Malhotra Pulkit
Department of Conservative Dentistry and Endodontics, Subharti Dental College, Meerut, Uttar Pradesh, India.
J Conserv Dent. 2016 Mar-Apr;19(2):179-83. doi: 10.4103/0972-0707.178705.
The purpose of this study was to evaluate the effect of phytic acid, ethylenediaminetetraacetic acid (EDTA), and chitosan solutions on the microhardness of human radicular dentin.
Thirty dentin specimens were randomly divided into three groups of 10 specimens each according to the irrigant used: G1 - 1% phytic acid, G2 - 17% EDTA, and G3 - 0.2% chitosan. A standardized volume of each chelating solution was used for 3 min. Dentin microhardness was measured before and after application at the cervical, middle, and apical levels with a Vickers indenter under a 200-g load and a 10-s dwell time. The results were analyzed using one-way analysis of variance (ANOVA) and Student's t test.
Microhardness of the radicular dentin varied at the cervical, middle, and apical levels. EDTA had the greatest overall effect, causing a sharp percentage reduction in dentin microhardness with a significant difference from phytic acid and chitosan (P = 0.002). However, phytic acid and chitosan differed insignificantly from each other (P = 0.887).
All tested chelating solutions reduced microhardness of the radicular dentin layer at all the levels. However, reduction was least at the apical level. EDTA caused more reduction in dentin microhardness than chitosan while phytic acid reduced the least.
本研究旨在评估植酸、乙二胺四乙酸（EDTA）和壳聚糖溶液对人牙根牙本质显微硬度的影响。
根据所使用的冲洗剂，将30个牙本质标本随机分为三组，每组10个标本：G1 - 1%植酸，G2 - 17% EDTA，G3 - 0.2%壳聚糖。每种螯合溶液使用标准化体积，作用3分钟。在200克载荷和10秒保压时间下，使用维氏压头在颈部、中部和根尖水平测量应用前后的牙本质显微硬度。结果采用单因素方差分析（ANOVA）和学生t检验进行分析。
牙根牙本质的显微硬度在颈部、中部和根尖水平有所不同。EDTA的总体影响最大，导致牙本质显微硬度百分比急剧下降，与植酸和壳聚糖有显著差异（P = 0.002）。然而，植酸和壳聚糖之间差异不显著（P = 0.887）。
所有测试的螯合溶液均降低了牙根牙本质层在所有水平的显微硬度。然而，根尖水平的降低最少。EDTA导致牙本质显微硬度的降低比壳聚糖更多，而植酸降低最少。
