来自紫狼尾草的一种高度分化的约翰逊草花叶病毒分离物的生物学和分子特征
Biological and molecular characterization of a highly divergent johnsongrass mosaic virus isolate from Pennisetum purpureum.
作者信息
Silva Karina N, Melo Fernando L, Orílio Anelise F, Nagata Tatsuya, Silva Marilia S, Fernandes Celso D, Fragoso Rodrigo R, Dessaune Suelen N, Resende Renato O
机构信息
Department of Cell Biology, University of Brasilia, Campus Universitário Darcy Ribeiro, Brasília, Distrito Federal, 70910-900, Brazil.
Embrapa Genetic Resources and Biotechnology, Brasília, Distrito Federal, Brazil.
出版信息
Arch Virol. 2016 Jul;161(7):1981-6. doi: 10.1007/s00705-016-2832-9. Epub 2016 Apr 21.
The complete genome sequence (9,865 nucleotides) of a highly divergent johnsongrass mosaic virus isolate (JGMV-CNPGL) was determined using Illumina sequencing. This isolate infected 10 genotypes of gramineous plants including maize. A comparative analysis of the complete genome showed 80 % nucleotide (nt) sequence identity (86 % amino acid (aa) sequence identity) to a johnsongrass mosaic virus isolate from Australia. The coat protein (CP) identity values, however, were lower than those for the whole genome (78 % and 80 % for nt and aa, respectively) and were close to the species demarcation values (77 % nt and 80 % aa). Unexpectedly, the amino-terminal portion of CP of JGMV-CNPGL showed only 38 % sequence identity to other JGMV isolates. The biological implications of this sequence divergence remain to be elucidated.
利用Illumina测序技术测定了一种高度分化的约翰逊草花叶病毒分离株（JGMV-CNPGL）的完整基因组序列（9865个核苷酸）。该分离株感染了包括玉米在内的10种禾本科植物基因型。对完整基因组的比较分析表明，其与来自澳大利亚的一种约翰逊草花叶病毒分离株具有80%的核苷酸（nt）序列同一性（86%的氨基酸（aa）序列同一性）。然而，外壳蛋白（CP）的同一性值低于全基因组的同一性值（nt和aa分别为78%和80%），且接近种的划分值（77% nt和80% aa）。出乎意料的是，JGMV-CNPGL的CP氨基末端部分与其他JGMV分离株的序列同一性仅为38%。这种序列差异的生物学意义仍有待阐明。
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