Yang Jing, Xi Kehu, Gui Yan, Wang Youhu, Zhang Fuhong, Ma Chunxia, Hong Hao, Liu Xiangyi, Meng Nannan, Zhang Xiaobing
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2015 Dec;29(23):2060-4.
To investigate 18β-sodium glycyrrhetinic acid impact on nasal mucosa epithelial cilia in rat models of allergic rhinitis (AR).
AR models were established by ovalbumin-induction. Wister rats were randomly divided into groups as normal group, model group, budesonide (0.2 mg/kg) group and sodium glycyrrhetinic acid (20 mg/kg and 40 mg/kg) group after the success of AR models. At 2 weeks and 4 weeks after treatment, the behavioral changes of rats were observed and recorded, and nasal septum mucosae were collected after 2 week and 4 week intervention, and the morphological changes of nasal mucosae were observed by electron microscope.
Model group developed typical AR symptoms, the total score in all animals was > 5. With budesonide and sodium glycyrrhetinic acid treatment, the AR symptoms were relieved, and the total scores were reduced significantly (P < 0.01). Compared with the model group: after 2 weeks' intervention, thick mucous secretions on the top of columnar epithelium cilia in rat nasal mucosa was significantly reduced, and cilia adhesion, lodging, shedding were relieved in budesonide group and sodium glycyrrhetinic acid group, the relieve in budesonide group was slightly better than that in sodium glycyrrhetinic acid group; after 4 week intervention, Cilia adhesion, lodging, shedding were completely vanished, and the cilia were ranged in regular direction in budesonide group and sodium glycyrrhetinic acid group. Cilia in sodium glycyrrhetinic acid (20 mg/kg) group was more orderly, smooth than that in budesonide group and sodium glycyrrhetinic acid group (40 mg/kg), and the condition of cilia in sodium glycyrrhetinic acid group (20 mg/kg) was similar to the normal group.
18β-sodium glycyrrhetinic acid is effective to restrain the pathological changes of nasal mucosa cilia in rat models of AR.
探讨18β-甘草次酸钠对变应性鼻炎（AR）大鼠模型鼻黏膜上皮纤毛的影响。
采用卵清蛋白诱导建立AR模型。AR模型成功建立后，将Wistar大鼠随机分为正常组、模型组、布地奈德（0.2mg/kg）组和甘草次酸钠（20mg/kg和40mg/kg）组。治疗2周和4周后，观察并记录大鼠的行为变化，干预2周和4周后采集鼻中隔黏膜，通过电子显微镜观察鼻黏膜的形态变化。
模型组出现典型的AR症状，所有动物的总评分>5。经布地奈德和甘草次酸钠治疗后，AR症状缓解，总评分显著降低（P<0.01）。与模型组相比：干预2周后，大鼠鼻黏膜柱状上皮纤毛顶端的黏稠分泌物明显减少，布地奈德组和甘草次酸钠组的纤毛黏附、倒伏、脱落情况得到缓解，布地奈德组的缓解情况略优于甘草次酸钠组；干预4周后，布地奈德组和甘草次酸钠组的纤毛黏附、倒伏、脱落情况完全消失，纤毛排列方向规则。甘草次酸钠（20mg/kg）组的纤毛比布地奈德组和甘草次酸钠组（40mg/kg）更有序、光滑，甘草次酸钠组（20mg/kg）的纤毛情况与正常组相似。
18β-甘草次酸钠对AR大鼠模型鼻黏膜纤毛的病理改变具有抑制作用。
