[产肌苷枯草芽孢杆菌ATCC 13952的全基因组测序与分析]
[Whole-genome sequencing and analysis of inosine- producing strain Bacillus subtilis ATCC 13952].
作者信息
Li Erhan, Yang Huilin, Wang Xiaolan, Wan Lingfei, Pan Hongmei, Zhu Du
出版信息
Wei Sheng Wu Xue Bao. 2015 Dec 4;55(12):1560-7.
OBJECTIVE
Bacillus subtilis ATCC 13952 is an inosine-producing strain. In order to study the mechanisms of inosine accumulation and offer help for molecular breeding, it is necessary to uncover the genome sequence of ATCC 13952.
METHODS
Whole-genome sequencing of ATCC 13952 is carried out by Solexa and Sanger sequencing. Genome assembly, gene prediction and functional annotation, GO/COG cluster analysis and synteny analysis are done using relevant software.
RESULTS
The complete genomic information of Bacillus subtilis ATCC 13952 is contained on a single circular chromosome of 3876276 bp with an average GC content of 45.8%. The genome sequence is deposited in the GenBank under the accession number CP009748. Comparative genomic analysis shows that ATCC 13952 should have significant genomic synteny with other Bacillus subtilis strains. On the other hand, some point mutation and deletions occurred in purine metabolism-related genes between ATCC 13952 and the standard strain.
CONCLUSION
The results of this study will provide a theoretical basis for subsequent further molecular breeding.
目的
枯草芽孢杆菌ATCC 13952是一株产肌苷的菌株。为了研究肌苷积累的机制并为分子育种提供帮助，有必要揭示ATCC 13952的基因组序列。
方法
采用Solexa和Sanger测序对ATCC 13952进行全基因组测序。使用相关软件进行基因组组装、基因预测与功能注释、GO/COG聚类分析和共线性分析。
结果
枯草芽孢杆菌ATCC 13952的完整基因组信息包含在一条3876276 bp的单环状染色体上，平均GC含量为45.8%。该基因组序列已存入GenBank，登录号为CP009748。比较基因组分析表明，ATCC 13952与其他枯草芽孢杆菌菌株应具有显著的基因组共线性。另一方面，ATCC 13952与标准菌株之间的嘌呤代谢相关基因发生了一些点突变和缺失。
结论
本研究结果将为后续进一步的分子育种提供理论依据。
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