[短小芽孢杆菌β-1,4-甘露聚糖酶的特性及在干酪乳杆菌中的异源表达]
[Characterization of β-1, 4-mannanase from Bacillus pumilus and heterologous expression in Lactobacillus casei].
作者信息
Zou Yexia, Lin Jinzhong, Bu Xuemei, Jiang Lulu, Chen Zhengjun, Ge Xiangyang
出版信息
Wei Sheng Wu Xue Bao. 2015 Dec 4;55(12):1576-83.
OBJECTIVE
Lactobacillus casei is widely used in food production and feed industry. The aim of this study was to construct the recombinant expression mannanase Lb. casei.
METHODS
The mature peptide gene of β-1,4-mannanase from Bacillus pumilus was cloned into expression vectors pELX1 and pELSH, then electroporated into Lb. casei, establishing an intracellular and a secretion expression mannanase Lb. casei respectively.
RESULTS
After incubation, the specific activity of β-1,4-mannanase was 23 U/mg whole cell protein for intracellular expression and 8.8 U/mL for secretion expression in supernatant.
CONCLUSION
Mannanase gene expression in Lb. casei provides application prospect and deserves further study.
目的
干酪乳杆菌广泛应用于食品生产和饲料工业。本研究旨在构建重组表达甘露聚糖酶的干酪乳杆菌。
方法
将短小芽孢杆菌β-1,4-甘露聚糖酶的成熟肽基因克隆到表达载体pELX1和pELSH中，然后电穿孔导入干酪乳杆菌，分别构建胞内表达和分泌表达甘露聚糖酶的干酪乳杆菌。
结果
培养后，胞内表达的β-1,4-甘露聚糖酶比活力为23 U/mg全细胞蛋白，分泌表达的上清液中比活力为8.8 U/mL。
结论
甘露聚糖酶基因在干酪乳杆菌中的表达具有应用前景，值得进一步研究。
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