放线菌素对人血清中肽酶所致内源性脑啡肽降解的保护作用。

Protection of endogenous enkephalin degradation from peptidases in human serum by actinonin.

作者信息

Nakajima S, Kaya K, Hazato T

机构信息

Department of Anesthesiology, School of Medicine, Juntendo University, Tokyo, Japan.

出版信息

Neuropeptides. 1989 Apr;13(3):201-6. doi: 10.1016/0143-4179(89)90093-0.

DOI:10.1016/0143-4179(89)90093-0

PMID:2710293

Abstract

This paper describes a new method for detecting the endogenous enkephalin level in human serum without interference from serum peptidases and contaminating proteins. The method consists of a combination of addition of a peptidase inhibitor, actinonin to serum and dialysis. The activity of enkephalin degrading enzymes was completely abolished by actinonin. Furthermore, contaminating proteins which interfered with RIA assay of enkephalins were removed by the dialysis process. This combination process enabled precise detection of the concentration of enkephalin-like substances in human serum. The mean normal serum level of leucine-enkephalin assayed by this method in 12 healthy volunteers was 93.4 pg/ml.

摘要

本文描述了一种检测人血清中内源性脑啡肽水平的新方法，该方法不受血清肽酶和污染蛋白的干扰。该方法包括向血清中添加肽酶抑制剂放线菌素，并结合透析。放线菌素可完全消除脑啡肽降解酶的活性。此外，通过透析过程去除了干扰脑啡肽放射免疫分析的污染蛋白。这种联合方法能够精确检测人血清中脑啡肽样物质的浓度。用该方法检测12名健康志愿者的亮氨酸脑啡肽平均正常血清水平为93.4 pg/ml。