Cornish S M, Wheal H V
Department of Neurophysiology, University of Southampton, U.K.
Neuroscience. 1989;28(3):563-71. doi: 10.1016/0306-4522(89)90005-5.
A paired pulse stimulation protocol was employed to examine the loss of inhibition in the hippocampus of the kainic acid-treated rat in vivo. Extracellular recordings were obtained from the CA1 pyramidal cell layer 1, 4, 8 and 16 weeks after a unilateral intracerebroventricular injection of kainic acid. Recordings were made both ipsilateral and contralateral to the site of kainic acid injection. The results demonstrated a loss of paired pulse inhibition in hippocampal CA1 area ipsilateral to the site of kainic acid injection which did not alter over 16 weeks. While the contralateral hippocampus showed no change 1 week after kainic acid injection, a reduction of paired pulse inhibition was seen after 4, 8 and 16 weeks. It is therefore apparent that the effects of kainic acid on the state of inhibition of the hippocampus are long-lasting and, furthermore, that the response to an injection of kainic acid is widespread, affecting the inhibitory control of the contralateral hippocampus.
采用配对脉冲刺激方案来检测在体给予 kainic 酸处理的大鼠海马中抑制作用的丧失。在单侧脑室内注射 kainic 酸后 1、4、8 和 16 周，从 CA1 锥体细胞层进行细胞外记录。记录在 kainic 酸注射部位的同侧和对侧进行。结果表明，在 kainic 酸注射部位同侧的海马 CA1 区配对脉冲抑制作用丧失，且在 16 周内未发生改变。虽然对侧海马在 kainic 酸注射后 1 周未显示变化，但在 4、8 和 16 周后可见配对脉冲抑制作用降低。因此，很明显 kainic 酸对海马抑制状态的影响是持久的，此外，对 kainic 酸注射的反应是广泛的，影响对侧海马的抑制控制。
