Saberi Kianoush, Gorji Mahlabani Mohammad Amin, Tashayoie Mohammad, Nasiri Nejad Farinaz
Anesthesiology Department, Imam Khomeini Medical and Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Under Graduate Department, Student's Scientific Research Center (SSRC), Exceptional Talent Development Center (ETDC), Tehran University of Medical Sciences, Tehran, Iran.
Anesth Pain Med. 2016 Feb 13;6(1):e32648. doi: 10.5812/aapm.32648. eCollection 2016 Feb.
Because the trend of pharmacotherapy is toward controlling diet rather than administration of drugs, in our study we examined the probable relationship between Creatine (Cr) or Whey (Wh) consumption and anesthesia (analgesia effect of ketamine). Creatine and Wh are among the most favorable supplements in the market. Whey is a protein, which is extracted from milk and is a rich source of amino acids. Creatine is an amino acid derivative that can change to ATP in the body. Both of these supplements result in Nitric Oxide (NO) retention, which is believed to be effective in N-Methyl-D-aspartate (NMDA) receptor analgesia.
The main question of this study was whether Wh and Cr are effective on analgesic and anesthetic characteristics of ketamine and whether this is related to NO retention or amino acids' features.
We divided 30 male Wistar rats to three (n = 10) groups; including Cr, Wh and sham (water only) groups. Each group was administered (by gavage) the supplements for an intermediate dosage during 25 days. After this period, they became anesthetized using a Ketamine-Xylazine (KX) and their time to anesthesia and analgesia, and total sleep time were recorded.
Data were analyzed twice using the SPSS 18 software with Analysis of Variance (ANOVA) and post hoc test; first time we expunged the rats that didn't become anesthetized and the second time we included all of the samples. There was a significant P-value (P < 0.05) for total anesthesia time in the second analysis. Bonferroni multiple comparison indicated that the difference was between Cr and Sham groups (P < 0.021).
The data only indicated that there might be a significant relationship between Cr consumption and total sleep time. Further studies, with rats of different gender and different dosage of supplement and anesthetics are suggested.
由于药物治疗的趋势是控制饮食而非给药，在我们的研究中，我们考察了服用肌酸（Cr）或乳清（Wh）与麻醉（氯胺酮的镇痛效果）之间可能存在的关系。肌酸和乳清是市场上最受欢迎的补充剂。乳清是一种从牛奶中提取的蛋白质，富含氨基酸。肌酸是一种氨基酸衍生物，在体内可转化为三磷酸腺苷（ATP）。这两种补充剂都会导致一氧化氮（NO）潴留，据信这对N-甲基-D-天冬氨酸（NMDA）受体镇痛有效。
本研究的主要问题是Wh和Cr是否对氯胺酮的镇痛和麻醉特性有效，以及这是否与NO潴留或氨基酸特性有关。
我们将30只雄性Wistar大鼠分为三组（每组n = 10只）；包括Cr组、Wh组和假手术组（仅给予水）。每组在25天内通过灌胃给予中等剂量的补充剂。在此期间后，使用氯胺酮-赛拉嗪（KX）使其麻醉，并记录其麻醉和镇痛时间以及总睡眠时间。
使用SPSS 18软件进行方差分析（ANOVA）和事后检验对数据进行了两次分析；第一次我们剔除了未麻醉的大鼠，第二次我们纳入了所有样本。在第二次分析中，总麻醉时间的P值具有显著性（P < 0.05）。Bonferroni多重比较表明，差异存在于Cr组和假手术组之间（P < 0.021）。
数据仅表明服用Cr与总睡眠时间之间可能存在显著关系。建议使用不同性别、不同补充剂和麻醉剂剂量的大鼠进行进一步研究。
