Murawski Marek, Woźniak Marta, Duś-Szachniewicz Kamila, Kołodziej Paweł, Rzeszutko Marta, Ziółkowski Piotr
Department of Gynecology and Obstetrics, Wrocław Medical University, Chałubińskiego 3, 50-368 Wrocław, Poland.
Department of Pathology, Wrocław Medical University, Marcinkowskiego 1, 50-368 Wrocław, Poland.
Int J Mol Sci. 2016 Apr 21;17(4):571. doi: 10.3390/ijms17040571.
One-step nucleic acid amplification (OSNA) detects and quantifies, with the use of a polymerase chain reaction, the presence of cytokeratin 19 mRNA in sentinel lymph nodes. The main advantage of the OSNA assay is the avoidance of second surgery in case of positive sentinel lymph node diagnosis. The objective of this study was to evaluate the significance of matrix metalloproteinase 9 expression by immunohistochemistry as supporting marker to cytokeratin 19 mRNA in sentinel lymph nodes in breast cancer patients and to relate this expression with clinicopathological data. This study was conducted on fresh sentinel lymph nodes obtained from 40 patients with tumors classified as carcinoma of no special type. The presence of metastatic cells in the slices of lymph nodes was evaluated by immunohistochemistry using antibodies for CK19 and MMP-9. Expression of CK19 and MMP-9 in lymph nodes was also confirmed by means of Western blot analysis. Results indicated that the strongest correlation with CK19 mRNA was displayed by MMP-9, CK19 (by immunohistochemistry, IHC), and nodal metastases (p < 0.001). Higher histological grading also positively correlated with CK19 mRNA, however that correlation was less significant. Since MMP-9 shows very strong correlation with CK19 mRNA in breast carcinoma of no special type metastases, expression of MMP-9 in sentinel lymph nodes should be considered as useful method whenever OSNA analysis is not available.
一步核酸扩增（OSNA）利用聚合酶链反应检测并定量前哨淋巴结中细胞角蛋白19信使核糖核酸的存在情况。OSNA检测的主要优势在于，在前哨淋巴结诊断呈阳性的情况下可避免二次手术。本研究的目的是评估通过免疫组织化学检测基质金属蛋白酶9表达作为乳腺癌患者前哨淋巴结中细胞角蛋白19信使核糖核酸支持标志物的意义，并将该表达与临床病理数据相关联。本研究对40例肿瘤分类为非特殊类型癌患者的新鲜前哨淋巴结进行。通过使用针对CK19和MMP-9的抗体进行免疫组织化学评估淋巴结切片中转移细胞的存在情况。还通过蛋白质印迹分析证实了淋巴结中CK19和MMP-9的表达。结果表明，MMP-9、CK19（通过免疫组织化学，IHC）和淋巴结转移与CK19信使核糖核酸显示出最强的相关性（p < 0.001）。较高的组织学分级也与CK19信使核糖核酸呈正相关，然而该相关性不太显著。由于在非特殊类型转移的乳腺癌中MMP-9与CK19信使核糖核酸显示出非常强的相关性，因此在前哨淋巴结中MMP-9的表达应被视为在无法进行OSNA分析时的一种有用方法。
