Carrera Inés, Richter Henning, Beckmann Katrin, Meier Dieter, Dennler Matthias, Kircher Patrick R
Am J Vet Res. 2016 May;77(5):452-62. doi: 10.2460/ajvr.77.5.452.
OBJECTIVE To investigate metabolite concentrations of the brains of dogs with intracranial neoplasia or noninfectious meningoencephalitis by use of short echo time, single voxel proton magnetic resonance spectroscopy ((1)H MRS) at 3.0 T. ANIMALS 29 dogs with intracranial lesions (14 with neoplasia [3 oligodendromas, 3 glioblastomas multiformes, 3 astrocytomas, 2 lymphomas, and 3 meningiomas] and 15 is with noninfectious meningoencephalitis) and 10 healthy control dogs. PROCEDURES Short echo time, single voxel (1)H-MRS at 3.0 T was performed on neoplastic and noninfectious inflammatory intracranial lesions identified with conventional MRI. Metabolites of interest included N-acetyl aspartate (NAA), total choline, creatine, myoinositol, the glutamine-glutamate complex (Glx), glutathione, taurine, lactate, and lipids. Data were analyzed with postprocessing fitting algorithm software. Metabolite concentrations relative to brain water content were calculated and compared with results for the healthy control dogs, which had been previously evaluated with the same (1)H MRS technique. RESULTS NAA, creatine, and Glx concentrations were reduced in the brains of dogs with neoplasia and noninfectious meningoencephalitis, whereas choline concentration was increased. Concentrations of these metabolites differed significantly between dogs with neoplasia and dogs with noninfectious meningoencephalitis. Concentrations of NAA, creatine, and Glx were significantly lower in dogs with neoplasia, whereas the concentration of choline was significantly higher in dogs with neoplasia. Lipids were predominantly found in dogs with high-grade intra-axial neoplasia, meningioma, and necrotizing meningoencephalitis. A high concentration of taurine was found in 10 of 15 dogs with noninfectious meningoencephalitis. CONCLUSIONS AND CLINICAL RELEVANCE (1)H MRS provided additional metabolic information about intracranial neoplasia and noninfectious meningoencephalitis in dogs.
目的 通过使用3.0T短回波时间单体素质子磁共振波谱（(1)H MRS）研究患有颅内肿瘤或非感染性脑膜脑炎犬的脑代谢物浓度。动物 29只患有颅内病变的犬（14只患有肿瘤[3只少突胶质细胞瘤、3只多形性胶质母细胞瘤、3只星形细胞瘤、2只淋巴瘤和3只脑膜瘤]和15只患有非感染性脑膜脑炎）以及10只健康对照犬。方法 对通过传统MRI识别出的肿瘤性和非感染性炎性颅内病变进行3.0T短回波时间单体素(1)H-MRS检查。感兴趣的代谢物包括N-乙酰天门冬氨酸（NAA）、总胆碱、肌酸、肌醇、谷氨酰胺-谷氨酸复合物（Glx）、谷胱甘肽、牛磺酸、乳酸和脂质。数据使用后处理拟合算法软件进行分析。计算相对于脑含水量的代谢物浓度，并与先前使用相同(1)H MRS技术评估的健康对照犬的结果进行比较。结果 患有肿瘤和非感染性脑膜脑炎犬的脑中NAA、肌酸和Glx浓度降低，而胆碱浓度升高。这些代谢物的浓度在患有肿瘤的犬和患有非感染性脑膜脑炎的犬之间存在显著差异。患有肿瘤的犬中NAA、肌酸和Glx的浓度显著较低，而患有肿瘤的犬中胆碱的浓度显著较高。脂质主要在高级别轴内肿瘤、脑膜瘤和坏死性脑膜脑炎的犬中发现。15只患有非感染性脑膜脑炎的犬中有10只发现高浓度牛磺酸。结论及临床意义 (1)H MRS为犬颅内肿瘤和非感染性脑膜脑炎提供了额外的代谢信息。
