Carrera Inés, Richter Henning, Meier Dieter, Kircher Patrick R, Dennler Matthias
Clinic of Diagnostic Imaging, Vetsuisse Faculty, University of Zurich, 8057 Zurich, Switzerland., Graduate School for Cellular and Biomedical Sciences, University of Bern, 3012 Bern, Switzerland.
Am J Vet Res. 2015 Feb;76(2):129-41. doi: 10.2460/ajvr.76.2.129.
To investigate regional differences of relative metabolite concentrations in the brain of healthy dogs with short echo time, single voxel proton magnetic resonance spectroscopy ((1)H MRS) at 3.0 T.
10 Beagles.
Short echo time, single voxel (1)H MRS was performed at the level of the right and left basal ganglia, right and left thalamus, right and left parietal lobes, occipital lobe, and cerebellum. Data were analyzed with an automated fitting method (linear combination model). Metabolite concentrations relative to water content were obtained, including N-acetyl aspartate, total choline, creatine, myoinositol, the sum of glutamine and glutamate (glutamine-glutamate complex), and glutathione. Metabolite ratios with creatine as the reference metabolite were calculated. Concentration differences between right and left hemispheres and sexes were evaluated with a Wilcoxon signed rank test and among various regions of the brain with an independent t test and 1-way ANOVA.
No significant differences were detected between sexes and right and left hemispheres. All metabolites, except the glutamine-glutamate complex and glutathione, had regional concentrations that differed significantly. The creatine concentration was highest in the basal ganglia and cerebellum and lowest in the parietal lobes. The N-acetyl aspartate concentration was highest in the parietal lobes and lowest in the cerebellum. Total choline concentration was highest in the basal ganglia and lowest in the occipital lobe.
Metabolite concentrations differed among brain parenchymal regions in healthy dogs. This study may provide reference values for clinical and research studies involving (1)H MRS performed at 3.0 T.
采用3.0 T短回波时间单体素质子磁共振波谱（(1)H MRS）研究健康犬脑内相对代谢物浓度的区域差异。
10只比格犬。
在左右基底神经节、左右丘脑、左右顶叶、枕叶和小脑水平进行短回波时间单体素(1)H MRS检查。采用自动拟合方法（线性组合模型）分析数据。获得相对于水含量的代谢物浓度，包括N-乙酰天门冬氨酸、总胆碱、肌酸、肌醇、谷氨酰胺和谷氨酸之和（谷氨酰胺 - 谷氨酸复合物）以及谷胱甘肽。计算以肌酸为参考代谢物的代谢物比率。采用Wilcoxon符号秩检验评估左右半球和不同性别之间的浓度差异，采用独立t检验和单因素方差分析评估脑内不同区域之间的浓度差异。
未检测到不同性别和左右半球之间存在显著差异。除谷氨酰胺 - 谷氨酸复合物和谷胱甘肽外所有代谢物的区域浓度均存在显著差异。肌酸浓度在基底神经节和小脑中最高，在顶叶中最低。N-乙酰天门冬氨酸浓度在顶叶中最高，在小脑中最低。总胆碱浓度在基底神经节中最高，在枕叶中最低。
健康犬脑实质区域的代谢物浓度存在差异。本研究可为涉及3.0 T (1)H MRS检查的临床和研究提供参考值。
