Qin Dao-feng, Li Ting, Dai Can
Ying Yong Sheng Tai Xue Bao. 2015 Dec;26(12):3865-70.
This study explored whether the degree of pollen limitation was affected by the experimental level (a single flower or inflorescence) and pollen quality (self-pollen or outcross-pollen) of supplemental pollination in Sagittaria trifolia. The results showed that the experimental level caused varying degree of pollen limitation. Compared with the inflorescence level, pollination at the single flower level led to a redistribution of resources among flowers, therefore affecting seed numbers. Pollen quality also played a vital role in the estimation of pollen limitation. Compared with self-pollen, supplemental pollination with outcross-pollen resulted in significantly more seeds and a higher germination rate. This proved that in the research system the reproduction was limited by pollen quality rather than quantity. Our study revealed that both experimental level and pollen quality had effects on the estimation of pollen limitation. It was suggested that in future studies we should evaluate pollen limitation at the inflorescence or whole plant level, and also consider comparing self- and outcross-pollen when applicable.
本研究探讨了野慈姑补充授粉的实验水平（单花或花序）和花粉质量（自花花粉或异花花粉）是否会影响花粉限制程度。结果表明，实验水平导致了不同程度的花粉限制。与花序水平相比，单花水平授粉导致花朵间资源重新分配，从而影响种子数量。花粉质量在花粉限制评估中也起着至关重要的作用。与自花花粉相比，异花花粉补充授粉产生的种子明显更多，发芽率更高。这证明在该研究系统中，繁殖受花粉质量而非数量的限制。我们的研究表明，实验水平和花粉质量均对花粉限制评估有影响。建议在未来研究中，应在花序或整株水平评估花粉限制，并且在适用时还应考虑比较自花花粉和异花花粉。
