凝乳多糖的热处理提高了生物活性β-(1,3)-葡聚糖低聚糖的酶法生产。
Heat treatment of curdlan enhances the enzymatic production of biologically active β-(1,3)-glucan oligosaccharides.
机构信息
Research Faculty of Agriculture, Hokkaido University, Kita-9 Nishi-9, Kita-ku, Sapporo 060-8589, Japan; Research Fellow of the Japan Society for the Promotion of Science (JSPS), 8 Ichibancho, Chiyoda-ku, Tokyo 102-8472, Japan.
Research Faculty of Agriculture, Hokkaido University, Kita-9 Nishi-9, Kita-ku, Sapporo 060-8589, Japan.
出版信息
Carbohydr Polym. 2016 Aug 1;146:396-401. doi: 10.1016/j.carbpol.2016.03.066. Epub 2016 Mar 24.
Biologically active β-(1,3)-glucan oligosaccharides were prepared from curdlan using GH64 enzyme (KfGH64). KfGH64 showed low activity toward native curdlan; thereby pretreatment conditions of curdlan were evaluated. KfGH64 showed the highest activity toward curdlan with heat treatment. The most efficient pretreatment (90°C for 0.5h) converted approximately 60% of curdlan into soluble saccharides under the optimized enzyme reaction conditions (pH 5.5, 37°C, 100rpm mixing speed, 24h, and 10μg of KfGH64/1g of curdlan). The resulting products were predominantly laminaripentaose and a small amount of β-(1,3)-glucans with an average degree of polymerization (DP) of 13 and 130. The products did not contain small oligosaccharides (DP<5), indicating that the hydrolysis of heat-treated curdlan by KfGH64 is a suitable method for the production of biologically active β-(1,3)-glucan oligosaccharides.
采用 GH64 酶（KfGH64）从卷枝霉素中制备具有生物活性的β-（1,3）-葡聚糖低聚糖。KfGH64 对天然卷枝霉素的活性较低；因此评估了卷枝霉素的预处理条件。KfGH64 对热处理的卷枝霉素表现出最高的活性。最有效的预处理（90°C 处理 0.5h）在优化的酶反应条件（pH5.5、37°C、100rpm 混合速度、24h 和 1g 卷枝霉素中加入 10μg 的 KfGH64）下，将约 60%的卷枝霉素转化为可溶性糖。所得产物主要为 laminaripentaose 和少量平均聚合度（DP）为 13 和 130 的β-（1,3）-葡聚糖。产物中不含低聚糖（DP<5），表明 KfGH64 水解热处理的卷枝霉素是生产具有生物活性的β-（1,3）-葡聚糖低聚糖的合适方法。
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