Krull U J, Brown R S, Koilpillai R N, Nespolo R, Safarzadeh-Amiri A, Vandenberg E T
Analyst. 1989 Jan;114(1):33-40. doi: 10.1039/an9891400033.
Interaction of the lectin concanavalin A with the polysaccharide glycogen can provide rapid spontaneous transients of the surface potential at bilayer and monolayer lipid membranes. The selective binding process can cause large, rapid potassium ion current fluctuations across bilayer membranes in a manner that is periodic and reproducible. The frequency of these transient ion current signals was shown to be related to sub-nanomolar concentrations of the reactive agents in aqueous solution. The physical mechanism responsible for ion current modulation was investigated by fluorescence methods using lipid vesicles, by the thermal dependence of the potassium ion current across planar bilayers and by pressure-area and dipolar potential measurements of lipid monolayers at an air-water interface. The mechanism is primarily associated with physical perturbations of lipid membranes by lectin-polysaccharide aggregates, resulting in the formation of localised domains of variable electrostatic potential and conductivity.
凝集素伴刀豆球蛋白A与多糖糖原的相互作用能够在双层和单层脂质膜上产生快速的表面电位自发瞬变。这种选择性结合过程能够以周期性且可重复的方式，引起跨双层膜的大幅快速钾离子电流波动。这些瞬态离子电流信号的频率与水溶液中反应剂的亚纳摩尔浓度有关。通过使用脂质囊泡的荧光方法、通过平面双层膜上钾离子电流的热依赖性以及通过气-水界面处脂质单层的压力-面积和偶极电位测量，研究了负责离子电流调制的物理机制。该机制主要与凝集素-多糖聚集体对脂质膜的物理扰动有关，导致形成具有可变静电势和电导率的局部区域。
