Mettler L, Salmassi A
Abteilung Frauenheilkunde im Zentrum für Operative Medizin I u. Michaelis-Hebammenschule, Universität zu Kiel, Bundesrepublik Deutschland.
Andrologia. 1989 Jan-Feb;21(1):55-65.
Immunizing Balb-c mice with washed motile human spermatozoa enabled the production of 2 monoclonal spermatozoal antibodies designated Ki-Sp II-13 and Ki-Sp VI-2. Immunohistochemically Ki-Sp VI-2 reacts only with mature spermatozoa. The monoclonal antibody Ki-Sp II-13 recognizes besides spermatozoa also T- and B-lymphocytes. Electronmicroscopically both antibodies react with the surface membrane of spermatozoa in the area of the head, the neck and the proximal part of the tail. Functional tests show a high sperm-agglutinating and sperm-immobilizing activity, using different test systems for Ki-Sp II-13. Both antibodies proved to be IgG. On immunoprecipitation Ki-Sp II-13 was identified as IgG-2a and Ki-Sp VI-2 as IgG1. Immunoprecipitates of SDS-electrophoretically separated and radiolabeled sperm surface antigens revealed a molecular weight of 18 KD for Ki-Sp II-13 and of 24 KD for Ki-Sp VI-2.
用洗涤过的活动人类精子免疫Balb-c小鼠，可产生两种单克隆精子抗体，分别命名为Ki-Sp II-13和Ki-Sp VI-2。免疫组织化学研究显示，Ki-Sp VI-2仅与成熟精子发生反应。单克隆抗体Ki-Sp II-13除了能识别精子外，还能识别T淋巴细胞和B淋巴细胞。电子显微镜观察表明，这两种抗体均与精子头部、颈部及尾部近端区域的表面膜发生反应。功能测试显示，使用针对Ki-Sp II-13的不同测试系统，其具有较高的精子凝集和精子制动活性。两种抗体均被证明为IgG。免疫沉淀实验表明，Ki-Sp II-13被鉴定为IgG-2a，Ki-Sp VI-2为IgG1。对SDS电泳分离并经放射性标记的精子表面抗原进行免疫沉淀，结果显示Ki-Sp II-13识别的抗原分子量为18 KD，Ki-Sp VI-2识别的抗原分子量为24 KD。
