Kaevska Marija, Videnska Petra, Sedlar Karel, Bartejsova Iva, Kralova Alena, Slana Iva
a Veterinary Research Institute, Hudcova 70, 62100 Brno, Czech Republic.
b Department of Biomedical Engineering, Brno University of Technology, Technicka 12, 61600 Brno, Czech Republic.
Can J Microbiol. 2016 Jun;62(6):538-41. doi: 10.1139/cjm-2015-0814. Epub 2016 Feb 29.
The aim of this study was to determine possible differences in the faecal microbiota of dairy cows infected with Mycobacterium avium subsp. paratuberculosis (Johne's disease) in comparison with noninfected cows from the same herds. Faecal samples from cows in 4 herds were tested for M. avium subsp. paratuberculosis by real-time PCR, and faecal bacterial populations were analysed by 454 pyrosequencing of the 16S rRNA gene. The most notable differences between shedding and nonshedding cows were an increase in the genus Psychrobacter and a decrease in the genera Oscillospira, Ruminococcus, and Bifidobacterium in cows infected with M. avium subsp. paratuberculosis. The present study is the first to report the faecal microbial composition in dairy cows infected with M. avium subsp. paratuberculosis.
本研究的目的是确定感染副结核分枝杆菌（约翰氏病）的奶牛与同一牛群中未感染奶牛的粪便微生物群可能存在的差异。对4个牛群中奶牛的粪便样本进行实时PCR检测副结核分枝杆菌，并通过16S rRNA基因的454焦磷酸测序分析粪便细菌种群。排菌奶牛和未排菌奶牛之间最显著的差异是，感染副结核分枝杆菌的奶牛中嗜冷杆菌属增加，而颤螺菌属、瘤胃球菌属和双歧杆菌属减少。本研究首次报告了感染副结核分枝杆菌的奶牛的粪便微生物组成。
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