Cháfer-Pericás C, Torres-Cuevas I, Sanchez-Illana A, Escobar J, Kuligowski J, Solberg R, Garberg H T, Huun M U, Saugstad O D, Vento M
Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain.
Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain.
Talanta. 2016 Jun 1;153:152-7. doi: 10.1016/j.talanta.2016.03.010. Epub 2016 Mar 4.
This paper describes a reliable analytical method based on ultra-performance liquid chromatography coupled to tandem mass spectrometry to determine F2-isoprostanes and other total byproducts (isoprostanes, isofurans, neuroprostanes and neurofurans) as lipid peroxidation biomarkers in newborn plasma samples. The proposed procedure is characterized by a simple sample treatment employing a reduced sample volume (100µL). Also, it shows a high throughput and high selectivity to determine simultaneously different isoprostane isomers in a large number of samples. The reliability of the described method was demonstrated by analysis of spiked plasma samples, obtaining recoveries between 70% and 130% for most of the analytes. Taking into account the implementation of further clinical studies, it was demonstrated the proper sensitivity of the method by means of the analysis of few human newborn plasma samples. In addition to this, newborn piglet plasma samples (n=80) were analyzed observing that the developed method was suitable to determine the analyte levels present in this kind of samples. Therefore, this analytical method could be applied in further clinical research about establishment of reliable lipid peroxidation biomarkers employing this experimental model.
本文描述了一种基于超高效液相色谱-串联质谱联用的可靠分析方法，用于测定新生儿血浆样本中作为脂质过氧化生物标志物的F2-异前列腺素及其他总副产物（异前列腺素、异呋喃、神经前列腺素和神经呋喃）。所提出的方法的特点是采用简单的样品处理方式，使用的样品体积减少（100µL）。此外，它在大量样品中同时测定不同异前列腺素异构体时具有高通量和高选择性。通过对加标血浆样本的分析证明了所述方法的可靠性，大多数分析物的回收率在70%至130%之间。考虑到进一步临床研究的实施，通过对少量人类新生儿血浆样本的分析证明了该方法具有适当的灵敏度。除此之外，对新生仔猪血浆样本（n = 80）进行了分析，观察到所开发的方法适用于测定这类样本中存在的分析物水平。因此，这种分析方法可应用于关于使用该实验模型建立可靠脂质过氧化生物标志物的进一步临床研究。
