Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain.
Department of Obstetrics and Gynecology, University of Helsinki, Helsinki University Hospital, Helsinki, Finland.
Anal Chim Acta. 2015 Jul 30;886:214-20. doi: 10.1016/j.aca.2015.06.028. Epub 2015 Aug 6.
Byproducts of arachidonic (AA) and docosahexaenoic acid (DHA) oxidation are highly relevant for the study of free radical associated conditions in the perinatal period. Plasma metabolites can provide the clinician with a snapshot of the oxidant status of patients before and after specific clinical interventions (e.g.: supplementation with oxygen). We describe a new andreliable ultra-performance liquid mass spectrometry method to determine F2-isoprostanes and other byproducts (isoprostanes, isofurans, neuroprostanes, neurofurans) in newborn serum samples. Cord blood samples were obtained from severely depressed newborn infants (Apgar score 1 min < 3; arterial cord pH < 7.00), and aliquoted for serum determination and stored at -80 °C. A UHPLC-MS/MS method was employed. It has a series of technical advantages: simple sample treatment; reduced sample volume (100 μL) which is essential for preterm neonates with low circulating blood volume, high throughput of sample analysis (96 samples in less than 24 h) and high selectivity for different isoprostanes isomers. Excellent sensitivity was achieved within limits of detection between 0.06 and 4.2 nmol L(-1), which renders this method suitable to monitoranalyte concentration in newborn samples. The method's precision was satisfactory; with coefficients of variation around 5-12% (intra-day) and 7-17% (inter-day). The reliability of the described method was assessed by analysis of spiked serum samples obtaining recoveries between 70% and 120%. The proposed method has rendered suitable for serum determination for newborn babies at risk of oxygen free radical associated conditions.
花生四烯酸 (AA) 和二十二碳六烯酸 (DHA) 氧化的副产物与围产期自由基相关条件的研究密切相关。血浆代谢物可以为临床医生提供患者在特定临床干预前后的氧化剂状态的快照（例如：补充氧气）。我们描述了一种新的可靠的超高效液相质谱法，用于测定新生儿血清样品中的 F2-类二十烷酸和其他副产物（类二十烷酸、异呋喃、神经前列腺烷酸、神经呋喃）。从严重抑郁的新生儿（Apgar 评分 1 分钟 < 3；动脉脐带 pH < 7.00）获得脐带血样本，并分成血清测定和储存在-80°C。采用 UHPLC-MS/MS 法。它具有一系列技术优势：简单的样品处理；减少样品量（100 μL），对于循环血量低的早产儿至关重要，样品分析的高通量（不到 24 小时可分析 96 个样品）和对不同的类二十烷酸异构体具有高选择性。在 0.06 到 4.2 nmol L(-1) 的检测限范围内实现了出色的灵敏度，这使得该方法适用于监测新生儿样品中的分析物浓度。该方法的精密度令人满意；日内变异系数约为 5-12%，日间变异系数约为 7-17%。通过分析加标血清样本评估了所描述方法的可靠性，回收率在 70%到 120%之间。该方法已适用于有发生氧自由基相关疾病风险的新生儿的血清测定。
