样品预处理对柠檬（Citrus limon）成分提取的影响。
Effect of sample pretreatment on the extraction of lemon (Citrus limon) components.
作者信息
Ledesma-Escobar Carlos A, Priego-Capote Feliciano, Luque de Castro María D
机构信息
Department of Analytical Chemistry, Annex C-3, Campus of Rabanales, University of Córdoba, E-14071 Córdoba, Spain; University of Córdoba Agroalimentary Excellence Campus, ceiA3, Campus of Rabanales, E-1407 Córdoba, Spain.
Department of Analytical Chemistry, Annex C-3, Campus of Rabanales, University of Córdoba, E-14071 Córdoba, Spain; University of Córdoba Agroalimentary Excellence Campus, ceiA3, Campus of Rabanales, E-1407 Córdoba, Spain; Maimónides Institute of Biomedical Research (IMIBIC), Reina Sofía Hospital, University of Córdoba, E-14071 Córdoba, Spain.
出版信息
Talanta. 2016 Jun 1;153:386-91. doi: 10.1016/j.talanta.2016.03.024. Epub 2016 Mar 9.
A study on the key role of lemon sample pretreatment on the analytical results is here presented. The objective of the study was to analyze the differences between extracts obtained from lyophilized and air-dried samples-the most common sample pretreatment in citrus studies-in comparison to extracts from fresh samples. All the extracts were obtained with ultrasound assistance and analyzed by LC-QTOF MS/MS. The dataset, constituted by 74 tentative identified metabolites, was first evaluated by ANOVA, which showed significant differences in the concentration of 44 out of 74 metabolites (p≤0.01). Also, the pairwise mean comparison (Tukey HSD; p≤0.01) revealed that the concentration of metabolites in the extracts from fresh and air-dried samples was quite similar and differed from that in lyophilized samples. On the other hand, application of principal component analysis (PCA) showed a clear discrimination between pretreatments, explaining 86.20% of the total variability. The results of this study suggest that the main differences between extracts could be attributed to the effect of freezing or heating on metabolic pathways, and not only to thermolability of the compounds.
本文介绍了一项关于柠檬样品预处理对分析结果的关键作用的研究。该研究的目的是分析冻干样品和风干样品（柑橘类研究中最常见的样品预处理方法）提取物与新鲜样品提取物之间的差异。所有提取物均通过超声辅助获得，并采用液相色谱-四极杆飞行时间串联质谱（LC-QTOF MS/MS）进行分析。由74种初步鉴定的代谢物组成的数据集首先通过方差分析进行评估，结果显示74种代谢物中有44种代谢物的浓度存在显著差异（p≤0.01）。此外，两两均值比较（Tukey HSD；p≤0.01）表明，新鲜样品和风干样品提取物中代谢物的浓度非常相似，与冻干样品中的浓度不同。另一方面，主成分分析（PCA）的应用显示出预处理之间有明显的区分，解释了总变异性的86.20%。这项研究的结果表明，提取物之间的主要差异可能归因于冷冻或加热对代谢途径的影响，而不仅仅是化合物的热稳定性。
Zotero 插件