脂质双层膜的二维¹H/¹³C异核化学位移相关光谱学。
Two-dimensional 1H/13C heteronuclear chemical shift correlation spectroscopy of lipid bilayers.
作者信息
Lee C W, Griffin R G
机构信息
Francis Bitter National Magnet Laboratory, Massachusetts Institute of Technology, Cambridge 02139.
出版信息
Biophys J. 1989 Feb;55(2):355-8. doi: 10.1016/S0006-3495(89)82812-7.
Abstract
Using solid-state magic angle spinning nuclear magnetic resonance (NMR) techniques, we have obtained two-dimensional (2D), 1H/13C chemical shift-correlated spectra of liquid crystalline 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC) bilayers in 30 wt% PO4/D2O buffer. Linewidths in both the 13C and the 1H dimensions were less than 0.3 ppm wide. The 2D spectrum consists of chemical shift correlations between all resolvable, directly bonded 13C-1H pairs and exhibits considerably greater spectral dispersion than either ID 1H or 13C MAS spectra. This approach promises to be an important tool in structural studies of biological membranes.
摘要
利用固态魔角旋转核磁共振（NMR）技术，我们获得了在30 wt% PO4/D2O缓冲液中液晶态1,2-二肉豆蔻酰-sn-甘油-3-磷酸胆碱（DMPC）双层膜的二维（2D）、1H/13C化学位移相关谱。13C和1H维度的线宽均小于0.3 ppm。二维谱由所有可分辨的直接键合的13C-1H对之间的化学位移相关性组成，并且与一维1H或13C MAS谱相比，具有显著更大的光谱分散性。这种方法有望成为生物膜结构研究中的一种重要工具。
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