Liu Lei, Wu Bin, Liu Ke, Li Chao-Ran, Zhou Xu, Li Ping, Yang Hua
State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing 210009, China.
State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing 210009, China.
J Pharm Biomed Anal. 2016 Jul 15;126:1-8. doi: 10.1016/j.jpba.2016.04.027. Epub 2016 Apr 21.
An ionic liquid (IL)-modified micellar electrokinetic chromatography (MEKC) method was proposed for the separation and determination of eight phenolic acids. In order to increase separation efficiency and selectivity, the micelle system consisting of aqueous mixtures of ILs, Tween 20 and borate was optimized using a D-optimal design. A 16-run experimental plan was carried out. The results indicated that the addition of ILs in background electrolyte could significantly alter the electrophoretic behavior and improve the resolution of target analytes. By evaluating the electropherograms obtained, a satisfactory separation condition for all analytes was achieved in 10min with optimized buffer composed of 0.70% (w/w) 1-butyl-3-methylimidazolium tetrafluoroborate, 8.1% (w/w) polyoxyethylene sorbitan monolaurate (Tween 20) and 10mM sodium borate at pH 9.2. Under these conditions, all calibration curves showed good linearity (r(2)>0.9969), and accuracy (recoveries ranging from 94.71 to 106.85%). Finally, the proposed method was successfully applied to determine the phenolic acids in a Chinese medicine compound, compound danshen dripping pills.
提出了一种离子液体（IL）修饰的胶束电动色谱（MEKC）方法用于分离和测定8种酚酸。为了提高分离效率和选择性，采用D-最优设计对由离子液体、吐温20和硼酸盐的水混合体系组成的胶束系统进行了优化。实施了一个16次运行的实验方案。结果表明，在背景电解质中加入离子液体可显著改变电泳行为并提高目标分析物的分离度。通过评估得到的电泳图，在由0.70%（w/w）四氟硼酸1-丁基-3-甲基咪唑鎓、8.1%（w/w）聚氧乙烯山梨醇酐单月桂酸酯（吐温20）和10 mM硼酸钠组成的优化缓冲液（pH 9.2）中，10分钟内实现了对所有分析物的满意分离条件。在这些条件下，所有校准曲线均显示出良好的线性（r²>0.9969）和准确度（回收率在94.71%至106.85%之间）。最后，所提出的方法成功应用于测定中药复方丹参滴丸中的酚酸。
