Castro Pedro Humberto, Verde Nuno, Tavares Rui Manuel, Bejarano Eduardo Rodríguez, Azevedo Herlânder
a BioSystems & Integrative Sciences Institute (BioISI), Plant Functional Biology Center, University of Minho, Campus de Gualtar , Braga , Portugal.
b Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora," Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC) , Dept. Biología Celular, Genética y Fisiología , Málaga , Spain.
Plant Signal Behav. 2018 Apr 3;13(4):e1179417. doi: 10.1080/15592324.2016.1179417. Epub 2018 Apr 16.
SUMO is a modifying peptide that regulates protein activity and is essential to eukaryotes. In plants, SUMO plays an important role in both development and the response to environmental stimuli. The best described sumoylation pathway component is the SUMO E3 ligase SIZ1. Its mutant displays inefficient responses to nutrient imbalance in phosphate, nitrate and copper. Recently, we reported that siz1 also displays altered responses to exogenous sugar supplementation. The siz1 mutant is a salicylic acid (SA) accumulator, and SA may interfere with sugar-dependent responses and signaling events. Here, we extended our previous studies to determine the importance of SA in the SIZ1 response to sugars, by introducing the bacterial salicylate hydroxylase NahG into the siz1 background. Results demonstrate that siz1 phenotypes involving delayed germination are partially dependent of SA levels, whereas the sugar-signaling effect of sugars is independent of SA.
SUMO是一种调节蛋白质活性的修饰肽，对真核生物至关重要。在植物中，SUMO在发育和对环境刺激的反应中都起着重要作用。描述得最为清楚的类泛素化途径成分是SUMO E3连接酶SIZ1。其突变体对磷酸盐、硝酸盐和铜的营养失衡反应效率低下。最近，我们报道siz1对外源糖补充的反应也发生了改变。siz1突变体是水杨酸（SA）积累者，SA可能会干扰糖依赖性反应和信号事件。在这里，我们通过将细菌水杨酸羟化酶NahG导入siz1背景，扩展了我们之前的研究，以确定SA在SIZ1对糖的反应中的重要性。结果表明，涉及发芽延迟的siz1表型部分依赖于SA水平，而糖的糖信号效应则独立于SA。
