Steimer T, Hutchison J B
Division of Biology of Growth and Reproduction, Faculty of Medicine, University of Geneva, Switzerland.
Brain Res. 1989 Feb 20;480(1-2):335-9. doi: 10.1016/0006-8993(89)90203-5.
Aromatization of testosterone in the preoptic area of the male ring dove is required for the expression of specific estrogen-sensitive sexual behavior. Estradiol metabolism and/or binding of the aromatization product(s) to the preoptic estrogen receptors (ER) could interfere with the apparent increase in estradiol formation that occurs after androgen administration to castrated males. Therefore, we have re-examined the induction mechanism using a direct in vitro assay of aromatase activity. Since levels of aromatase activity and enzyme kinetics were similar irrespective of the assay used, we can conclude that increased estradiol formation after androgen stimulation is mainly due to enzyme induction and that estrogen binding and/or metabolism do not interfere significantly with the induction process.
雄性环鸽视前区睾酮的芳香化作用是特定雌激素敏感性性行为表达所必需的。雌二醇代谢和/或芳香化产物与视前区雌激素受体（ER）的结合可能会干扰给去势雄性动物注射雄激素后雌二醇形成的明显增加。因此，我们使用芳香化酶活性的直接体外测定法重新研究了诱导机制。由于无论使用何种测定方法，芳香化酶活性水平和酶动力学都是相似的，我们可以得出结论，雄激素刺激后雌二醇形成增加主要是由于酶诱导，并且雌激素结合和/或代谢不会对诱导过程产生显著干扰。
