Kazama Daisuke, Itakura Masateru, Kurusu Takamitsu, Mitsuda Nobutaka, Ohme-Takagi Masaru, Tada Yuichi
Graduate School of Bionics, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, Japan.
School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, Japan.
Plants (Basel). 2013 Dec 5;2(4):769-85. doi: 10.3390/plants2040769.
We produced transgenic Arabidopsis plants that express chimeric genes for transcription factors converted to dominant repressors, using Chimeric REpressor gene-Silencing Technology (CRES-T), and evaluated the salt tolerance of each line. The seeds of the CRES-T lines for ADA2b, Msantd, DDF1, DREB26, AtGeBP, and ATHB23 exhibited higher germination rates than Wild type (WT) and developed rosette plants under up to 200 mM NaCl or 400 mM mannitol. WT plants did not grow under these conditions. In these CRES-T lines, the expression patterns of stress-related genes such as RD29A, RD22, DREB1A, and P5CS differed from those in WT plants, suggesting the involvement of the six transcription factors identified here in the stress response pathways regulated by the products of these stress-related genes. Our results demonstrate additional proof that CRES-T is a superior tool for revealing the function of transcription factors.
我们利用嵌合抑制基因沉默技术（CRES-T）培育了表达转化为显性抑制因子的转录因子嵌合基因的转基因拟南芥植株，并评估了每个株系的耐盐性。ADA2b、Msantd、DDF1、DREB26、AtGeBP和ATHB23的CRES-T株系种子的发芽率高于野生型（WT），并且在高达200 mM NaCl或400 mM甘露醇条件下能发育出莲座状植株。WT植株在这些条件下无法生长。在这些CRES-T株系中，与胁迫相关的基因如RD29A、RD22、DREB1A和P5CS的表达模式与WT植株不同，这表明这里鉴定出的六个转录因子参与了由这些胁迫相关基因的产物调控的胁迫反应途径。我们的结果进一步证明CRES-T是揭示转录因子功能的优越工具。
