草药冬虫夏草可保护胰岛β细胞免受链脲佐菌素诱导的内质网应激。
The Herbal Medicine Cordyceps sinensis Protects Pancreatic Beta Cells from Streptozotocin-Induced Endoplasmic Reticulum Stress.
机构信息
Department of Physiology, North Sichuan Medical College, Nanchong, Sichuan, China.
Department of Pharmacology, North Sichuan Medical College, Nanchong, Sichuan, China.
出版信息
Can J Diabetes. 2016 Aug;40(4):329-35. doi: 10.1016/j.jcjd.2016.02.001. Epub 2016 Apr 29.
OBJECTIVES
Our previous work found that Cordyceps sinensis (CS) improves the activity and secretory function of pancreatic islet beta cells. The objective was to observe a further possible role of CS in the protection of insulin-secreting cells.
METHODS
A rat model of type 2 diabetes mellitus was developed with streptozotocin (STZ) and a high-energy fat diet (HFD). CS was administered in the successful model of rats with type 2 diabetes. After 4 weeks, the biochemistry index of blood samples was measured, and pathologic observation was performed by immunohistochemistry.
RESULTS
In the rats with type 2 diabetes induced by a HFD and STZ, the levels of fasting blood glucose and fasting insulin were elevated, and the insulin sensitivity index was decreased. Pathologic examination found an increased number of apoptotic cells, an elevated protein expression of pro-apoptotic C/EBP homologous protein (CHOP) and an increased c-Jun level by means of JNK phosphorylation, responsive to the endoplasmic reticulum stress of islet beta cells. With treatment by CS for 4 weeks, the elevated levels of both fasting blood glucose and fasting insulin in the rats with type 2 diabetes were significantly lower, and the decreased insulin sensitivity index was reversed. Compared to the control rats with type 2 diabetes, CS application significantly reduced the number of apoptotic cells and decreased protein expression of both CHOP and c-Jun.
CONCLUSIONS
The herbal compound CS could protect pancreatic beta cells from the pro-apoptotic endoplasmic reticulum stress induced by HFD-STZ. This suggests an alternative approach to treating type 2 diabetes.
目的
我们之前的工作发现，蛹虫草（CS）可提高胰岛β细胞的活性和分泌功能。本研究旨在进一步观察 CS 对胰岛素分泌细胞的保护作用。
方法
采用链脲佐菌素（STZ）和高脂饮食（HFD）诱导建立 2 型糖尿病大鼠模型，用 CS 对成功建立的 2 型糖尿病大鼠模型进行干预。4 周后，检测血生化指标，采用免疫组化法进行病理观察。
结果
在 HFD-STZ 诱导的 2 型糖尿病大鼠中，空腹血糖和空腹胰岛素水平升高，胰岛素敏感指数降低。病理检查发现，胰岛β细胞内质网应激导致凋亡细胞增多，促凋亡转录因子 C/EBP 同源蛋白（CHOP）蛋白表达升高，c-Jun 通过 JNK 磷酸化水平升高。用 CS 治疗 4 周后，2 型糖尿病大鼠的空腹血糖和空腹胰岛素水平显著降低，胰岛素敏感指数降低得到逆转。与 2 型糖尿病对照大鼠相比，CS 应用显著减少了凋亡细胞数量，降低了 CHOP 和 c-Jun 的蛋白表达。
结论
中草药复方 CS 可保护胰岛β细胞免受 HFD-STZ 诱导的促凋亡内质网应激，为治疗 2 型糖尿病提供了一种新的方法。
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