Segura Elodie
INSERM U932, and Institut Curie, Centre de recherche, 26 rue d'Ulm, 75248, Paris Cedex 05, France.
Methods Mol Biol. 2016;1423:189-98. doi: 10.1007/978-1-4939-3606-9_14.
The presentation of exogenous antigens on major histocompatibility complex (MHC) class I molecules, termed cross-presentation, is essential for the initiation of cytotoxic immune responses. Numerous studies in mice and human have shown that dendritic cells are the best cross-presenting cells. The protocol described here allows the assessment of the cross-presentation by human dendritic cells of a model antigen (either soluble or cell associated) to antigen-specific CD8 T cells.
外源性抗原在主要组织相容性复合体（MHC）I类分子上的呈递，即交叉呈递，对于启动细胞毒性免疫反应至关重要。在小鼠和人类中进行的大量研究表明，树突状细胞是最佳的交叉呈递细胞。本文所述的方案可用于评估人类树突状细胞将模型抗原（可溶性或细胞相关）交叉呈递给抗原特异性CD8 T细胞的情况。
