无堆积胶的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳：用于高样品通量。
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis without a stacking gel: use for high sample throughput.
作者信息
Marshall T, Gaston S J, Williams K M
机构信息
Biology Department, The University of Ulster, Coleraine, Northern Ireland.
出版信息
Electrophoresis. 1989 Jan;10(1):63-5. doi: 10.1002/elps.1150100115.
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis without a stacking gel minimizes lateral spreading of protein when samples are applied in agarose wells and allows high sample throughput (6 samples/cm gel width). The method is simple and convenient to use and gives comparable resolution to the standard method with 4-20% or 6-30% polyacrylamide gradient gels. Best results are obtained when the upper zone of the separating gel is of low polyacrylamide concentration. This indicates a need for the molten agarose to penetrate and anneal with the separating gel.
不使用堆积胶的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，在将样品加样于琼脂糖孔中时可最大程度减少蛋白质的横向扩散，并可实现高样品通量（每厘米凝胶宽度6个样品）。该方法使用简单便捷，与使用4-20%或6-30%聚丙烯酰胺梯度凝胶的标准方法具有相当的分辨率。当分离胶的上部区域为低聚丙烯酰胺浓度时可获得最佳结果。这表明需要使熔融琼脂糖渗透并与分离胶退火。
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