高纯度琼脂糖作为十二烷基硫酸钠/聚丙烯酰胺凝胶电泳中的堆积胶。
Highly purified agarose as stacking gel in sodium dodecyl sulphate/polyacrylamide-gel electrophoresis.
作者信息
Aasted B
出版信息
Biochem J. 1980 Jul 1;189(1):183-4. doi: 10.1042/bj1890183.
Abstract
A modified sodium dodecyl sulphage/polyacrylamide-gel-electrophoretic method is described that utilizes highly purified agarose as stacking gel. The same electrophoretic resolution of different marker proteins is found as when polyacrylamide is used as stacking gel, but the background staining seen when polyacrylamide is used as stacking gel is decreased.
摘要
本文描述了一种改良的十二烷基硫酸钠/聚丙烯酰胺凝胶电泳方法，该方法使用高度纯化的琼脂糖作为堆积凝胶。与使用聚丙烯酰胺作为堆积凝胶时相比，不同标记蛋白的电泳分辨率相同，但使用聚丙烯酰胺作为堆积凝胶时出现的背景染色减少。
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Highly purified agarose as stacking gel in sodium dodecyl sulphate/polyacrylamide-gel electrophoresis.高纯度琼脂糖作为十二烷基硫酸钠/聚丙烯酰胺凝胶电泳中的堆积胶。
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本文引用的文献
1
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
2
Agarose isoelectric focusing of native human immunoglobulin M and alpha 2-macroglobulin.天然人免疫球蛋白M和α2-巨球蛋白的琼脂糖等电聚焦
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