Almeida Ana Luiza Normanha Ribeiro de, Bernardes Mário Vinícius Angelete Alvarez, Feitosa Marley Ribeiro, Peria Fernanda Maris, Tirapelli Daniela Pretti da Cunha, Rocha José Joaquim Ribeiro da, Feres Omar
Ribeirão Preto Medical School, University of São Paulo, Brazil.
Department of Surgery and Anatomy, Ribeirão Preto Medical School, University of São Paulo, Brazil.
Acta Cir Bras. 2016;31 Suppl 1:13-8. doi: 10.1590/S0102-86502016001300004.
This paper describes the ability of miRNA value predict oncological outcomes in CRC patients and correlates to clinical and pathologic variables.
We prospectively analyzed the serological expression of microRNA-21, microRNA-34a, and microRNA-126 in 37 stage II - IV CRC patients and correlate to seven fit counterparts. Serological microRNAs were extracted using the miRNeasy Mini Kit(r) (Qiagen, Hilden, Germany). Quantification of microRNAs was performed using TaqMan Master Mix(r) reagent (Applied Biosystems, USA).
We obtained serological underexpression microRNA-21, microRNA-34a, and microRNA-126 in CRC group. However, miRNAs serological values do not impact prognosis. Furthermore, miRNAs was not influenced by CEA values, TNM staging, and histological subtype.
Despite lower expression of miR-21, miR-34a and miR-126 in the CRC group, no association with poor prognosis was found.
本文描述了微小RNA值预测结直肠癌患者肿瘤学结局的能力，并与临床和病理变量相关联。
我们前瞻性分析了37例II-IV期结直肠癌患者血清中微小RNA-21、微小RNA-34a和微小RNA-126的表达情况，并与7例健康对照者进行了对比。使用miRNeasy Mini Kit（德国希尔德市Qiagen公司）提取血清中的微小RNA。使用TaqMan Master Mix试剂（美国应用生物系统公司）对微小RNA进行定量分析。
我们发现结直肠癌组血清中微小RNA-21、微小RNA-34a和微小RNA-126表达下调。然而，微小RNA的血清学值并不影响预后。此外，微小RNA不受癌胚抗原值、TNM分期和组织学亚型的影响。
尽管结直肠癌组中miR-21、miR-34a和miR-126表达较低，但未发现与预后不良相关。
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