Yamamoto Y, Osawa A, Inoue Y, Chûjô R, Suzuki T
Department of Biomolecular Engineering, Tokyo Institute of Technology, Japan.
FEBS Lett. 1989 Apr 24;247(2):263-7. doi: 10.1016/0014-5793(89)81349-3.
2D NMR spectroscopies have been successfully used to characterize the heme peripheral vinyl groups in paramagnetic hemoprotein in spite of the difficulties from the rapid paramagnetic relaxation and the low digital resolution of the 2D NMR map. The scalar coupling network system among the vinyl protons is clearly identified in the COSY spectra from its characteristic cross-peak pattern and the dipolar coupling connectivities of the vinyl proton resonances with other heme side-chain proton resonances not only provide the specific assignment of vinyl beta-proton resonances but also allow the determination of the vinyl group orientation with respect to the heme plane.
尽管存在顺磁弛豫速度快和二维核磁共振图谱数字分辨率低等困难，但二维核磁共振光谱已成功用于表征顺磁性血红蛋白中血红素外周乙烯基。乙烯基质子之间的标量耦合网络系统在化学位移相关谱（COSY）中通过其特征交叉峰模式清晰可辨，乙烯基质子共振与其他血红素侧链质子共振的偶极耦合连接不仅能对乙烯基β-质子共振进行特定归属，还能确定乙烯基相对于血红素平面的取向。
