Chen Xiaojing, Yang Xiaoqing, Wu Rongrong, Chen Weiwei, Xie Huihui, Qian Xia, Zhang Yuquan
Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Nantong, China.
Department of Gynecology, Tumor Hospital of Nantong, Nantong, China.
J Obstet Gynaecol Res. 2016 Aug;42(8):972-82. doi: 10.1111/jog.12984. Epub 2016 May 5.
The aim of this study was to investigate the therapeutic effects and mechanism of Wharton jelly-derived mesenchymal stem cells (WJ-MSC) in a spontaneous-abortion rat model.
An abortion model was established using intravenous injection of bromocriptine. WJ-MSC were isolated and cultured from Wharton jelly of the human umbilical cord. The pathologic changes of placenta decidual tissues were observed after hematoxylin-eosin staining. The embryo absorption rate was counted. The protein and mRNA levels of interleukin (IL)-10, interferon (IFN)-γ and IL-17 in each group were tested by enzyme-linked immunosorbent assay, Western blot and quantitative real-time polymerase chain reaction.
After bromocriptine injection, decidual cells degenerated and the connections between them loosened. Furthermore, some of the decidual cells were necrotic and the nuclei had disappeared. However, the transplantation of a large population of WJ-MSC prevented these damages from occurring. The changes of levels of IL-10, IFN-γ and IL-17 in serum and placenta decidual tissues induced by bromocriptine were well restored by a high, but not a low, dose of WJ-MSC engraft.
WJ-MSC can ameliorate early pregnancy loss. The mechanism may be related to its upregulation of IL-10 and downregulation of IFN-γ and IL-17.
本研究旨在探讨脐带来源的间充质干细胞（WJ-MSC）对自然流产大鼠模型的治疗作用及机制。
采用静脉注射溴隐亭建立流产模型。从人脐带华通胶中分离培养WJ-MSC。苏木精-伊红染色后观察胎盘蜕膜组织的病理变化。计算胚胎吸收率。通过酶联免疫吸附测定、蛋白质印迹法和定量实时聚合酶链反应检测每组中白细胞介素（IL）-10、干扰素（IFN）-γ和IL-17的蛋白质和mRNA水平。
注射溴隐亭后，蜕膜细胞退变，细胞间连接松散。此外，部分蜕膜细胞坏死，细胞核消失。然而，大量WJ-MSC的移植可防止这些损伤的发生。高剂量而非低剂量的WJ-MSC移植能很好地恢复溴隐亭诱导的血清和胎盘蜕膜组织中IL-10、IFN-γ和IL-17水平的变化。
WJ-MSC可改善早期妊娠丢失。其机制可能与其上调IL-10以及下调IFN-γ和IL-17有关。
