Schriever F, Dennis R D, Pallmann B, Riethmüller G, Johnson J P
Institute of Immunology, University of Munich, West Germany.
Hybridoma. 1989 Apr;8(2):153-9. doi: 10.1089/hyb.1989.8.153.
The present study investigates the chemical structure of a ganglioside, detected by monoclonal antibody (MAb) MacG1, which reacts with intracytoplasmic granules of tumor-infiltrating macrophages. The results obtained by enzymatic hydrolysis and fast-atom bombardment-mass spectrometry reveal that MAb MacG1 reacts with a subcomponent of the ganglioside GM3 found in melanoma and bovine brain. MAb MacG1 might be a powerful tool to distinguish among GM3 species and could help to define their possibly different biological functions.
本研究调查了一种神经节苷脂的化学结构，该神经节苷脂由单克隆抗体（MAb）MacG1检测到，它与肿瘤浸润巨噬细胞的胞浆颗粒发生反应。通过酶促水解和快原子轰击质谱法获得的结果表明，MAb MacG1与黑色素瘤和牛脑中发现的神经节苷脂GM3的一个亚组分发生反应。MAb MacG1可能是区分GM3种类的有力工具，并有助于确定它们可能不同的生物学功能。
