He Jian Wei, Hassan Yousef I, Perilla Norma, Li Xiu-Zhen, Boland Greg J, Zhou Ting
Guelph Research and Development Centre, Agriculture and Agri-Food Canada, GuelphON, Canada; School of Environmental Sciences, University of Guelph, GuelphON, Canada.
Guelph Research and Development Centre, Agriculture and Agri-Food Canada, Guelph ON, Canada.
Front Microbiol. 2016 Apr 21;7:572. doi: 10.3389/fmicb.2016.00572. eCollection 2016.
Deoxynivalenol (DON) is a toxic secondary metabolite produced by several Fusarium species that infest wheat and corn. Food and feed contaminated with DON pose a health risk to both humans and livestock and form a major barrier for international trade. Microbial detoxification represents an alternative approach to the physical and chemical detoxification methods of DON-contaminated grains. The present study details the characterization of a novel bacterium, Devosia mutans 17-2-E-8, that is capable of transforming DON to a non-toxic stereoisomer, 3-epi-deoxynivalenol under aerobic conditions, mild temperature (25-30°C), and neutral pH. The biotransformation takes place in the presence of rich sources of organic nitrogen and carbon without the need of DON to be the sole carbon source. The process is enzymatic in nature and endures a high detoxification capacity (3 μg DON/h/10(8) cells). The above conditions collectively suggest the possibility of utilizing the isolated bacterium as a feed treatment to address DON contamination under empirical field conditions.
脱氧雪腐镰刀菌烯醇（DON）是由几种侵染小麦和玉米的镰刀菌属产生的有毒次生代谢产物。被DON污染的食品和饲料对人类和牲畜都构成健康风险，并成为国际贸易的主要障碍。微生物解毒是一种替代DON污染谷物物理和化学解毒方法的途径。本研究详细描述了一种新型细菌——变形德沃斯氏菌17-2-E-8的特性，该细菌能够在有氧条件、温和温度（25-30°C）和中性pH值下将DON转化为无毒的立体异构体3-表脱氧雪腐镰刀菌烯醇。生物转化在富含有机氮和碳源的情况下发生，无需DON作为唯一碳源。该过程本质上是酶促反应，具有较高的解毒能力（3μg DON/小时/10⁸个细胞）。上述条件共同表明，在实际田间条件下，利用分离出的细菌作为饲料处理方法来解决DON污染问题具有可能性。
