Bose R
Department of Obstetrics, Gynecology and Reproductive Sciences, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
Immunol Lett. 1989 Mar;20(4):261-7. doi: 10.1016/0165-2478(89)90033-3.
The present study demonstrates that embryo associated immunosuppressive factor(s) (EASF) secreted by the human embryo at pre- and post-implantation stages share some similarities. Human EASF was partially purified from embryo growth media of in vitro fertilized ova and from first trimester pregnancy sera. Non-pregnancy sera were fractionated in parallel. During each step of purification the fractions were tested for immunosuppressive properties using concanavalin A-stimulated lymphocyte proliferation assay. Analysis of EASF positive fractions on SDS-PAGE identified 14 kDa and 24 kDa molecules in embryo growth media and pregnancy sera. No such molecules were found in control sera, suggesting that these factors are embryo associated. The relationship of pre- and post-implantation EASF was also analyzed by EASF binding assay using murine anti-EASF antibody, which was raised against EASF isolated from embryo growth media. Results show that murine antibody bound to EASF purified from pregnancy sera, but not to identical fractions from control sera, indicating that these post-implantation EASF possess some similarity with pre-implantation EASF. Results also indicate that a species of suppressor factors present in embryo growth media and pregnancy sera were unique for their origin. Presence of these three EASFs at various stages of gestation may play a role in suppressing maternal cellular immune responses thereby preventing maternal rejection of the embryo.
本研究表明，人类胚胎在着床前和着床后阶段分泌的胚胎相关免疫抑制因子（EASF）具有一些相似性。人EASF是从体外受精卵子的胚胎生长培养基和孕早期血清中部分纯化得到的。同时对非妊娠血清进行分级分离。在纯化的每个步骤中，使用伴刀豆球蛋白A刺激的淋巴细胞增殖试验检测各分级分离物的免疫抑制特性。对EASF阳性分级分离物进行SDS-PAGE分析，在胚胎生长培养基和妊娠血清中鉴定出14 kDa和24 kDa的分子。在对照血清中未发现此类分子，表明这些因子与胚胎相关。还使用针对从胚胎生长培养基中分离的EASF产生的鼠抗EASF抗体，通过EASF结合试验分析了着床前和着床后EASF的关系。结果表明，鼠抗体与从妊娠血清中纯化的EASF结合，但不与对照血清中的相同分级分离物结合，表明这些着床后EASF与着床前EASF具有一些相似性。结果还表明，胚胎生长培养基和妊娠血清中存在的一种抑制因子在其来源上是独特的。这三种EASF在妊娠各阶段的存在可能在抑制母体细胞免疫反应从而防止母体排斥胚胎方面发挥作用。
