2012年在越南流行的肠道病毒71型毒株的基因特征分析。
Genetic characterization of Enterovirus 71 strains circulating in Vietnam in 2012.
作者信息
Donato Celeste, Hoi Le Thi, Hoa Nguyen Thi, Hoa Tran Mai, Van Duyet Le, Dieu Ngan Ta Thi, Van Kinh Nguyen, Vu Trung Nguyen, Vijaykrishna Dhanasekaran
机构信息
Duke-NUS Medical School, 8 College Rd, Singapore.
National Hospital of Tropical Diseases, 78 Giai Phong Street, Hanoi, Vietnam.
出版信息
Virology. 2016 Aug;495:1-9. doi: 10.1016/j.virol.2016.04.026. Epub 2016 May 3.
BACKGROUND
Enterovirus 71 subgenogroup C4 caused the largest outbreak of Hand, Foot and Mouth Disease (HFMD) in Vietnam during 2011-2012, resulting in over 200,000 hospitalisations and 207 fatalities.
METHODS
A total of 1917 samples with adequate volume for RT-PCR analysis were collected from patients hospitalised with HFMD throughout Vietnam and 637 were positive for EV71. VP1 gene (n=87) and complete genome (n=9) sequencing was performed. Maximum-likelihood phylogenetic analysis was performed to characterise the B5, C4 and C5 strains detected.
RESULTS
Sequence analyses revealed that the dominant subgenogroup associated with the 2012 outbreak was C4, with B5 and C5 strains representing a small proportion of these cases.
CONCLUSIONS
Numerous countries in the region including Malaysia, Taiwan and China have a large influence on strain diversity in Vietnam and understanding the transmission of EV71 throughout Southeast Asia is vital to inform preventative public health measures and vaccine development efforts.
背景
肠道病毒71型C4亚基因组在2011 - 2012年期间引发了越南最大规模的手足口病（HFMD）疫情，导致超过20万例住院病例和207人死亡。
方法
从越南各地因手足口病住院的患者中总共收集了1917份体积足够用于逆转录聚合酶链反应（RT-PCR）分析的样本，其中637份肠道病毒71型呈阳性。对87份样本进行了VP1基因测序，对9份样本进行了全基因组测序。进行了最大似然系统发育分析，以鉴定检测到的B5、C4和C5毒株。
结果
序列分析显示，与2012年疫情相关的主要亚基因组是C4，B5和C5毒株在这些病例中占比很小。
结论
该地区包括马来西亚、台湾和中国在内的许多国家对越南毒株的多样性有很大影响，了解肠道病毒71型在整个东南亚的传播情况对于制定预防性公共卫生措施和疫苗研发工作至关重要。
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