[干扰素对黑色素瘤细胞的体外生长抑制作用。与重组干扰素α-2a相比，天然干扰素β浓度低100 - 1000倍时具有相似的抗增殖作用]
[Growth inhibition of melanoma cells by interferons in vitro. Similar antiproliferative effects of 100-1000 times lower concentrations of nIFN-beta in comparison with rIFN-alpha-2a].
作者信息
Zouboulis C, Garbe C, Orfanos C E
机构信息
Universitäts-Hautklinik, Freien Universität Berlin.
出版信息
Hautarzt. 1989 Feb;40(2):65-9.
The type I interferons (IFNs) rIFN-alpha 2a and nIFN-beta showed a significant dose-dependent antiproliferative effect on melanoma cell monolayer cultures in vitro compared with untreated controls (P less than 0.01 at 10 IU/ml; P less than 0.01 and P less than 0.001 at 1,000 IU/ml). The antiproliferative effect of a single IFN dose lasted for a maximum of 4 days, after which uninhibited proliferation of melanoma cells was again observed. In all experiments nIFN-beta showed a similar antiproliferative effect to rIFN-alpha 2a on the two melanoma cell lines (StML-11, SKMel-28) tested, when used in concentrations 100-1,000 times lower. At concentrations of 10,000 IU/ml rIFN-alpha 2a and 100-1,000 IU/ml hIFN-beta cytotoxic effects were observed.
与未处理的对照相比，I型干扰素（IFN）重组干扰素α2a和天然干扰素β在体外对黑色素瘤细胞单层培养物显示出显著的剂量依赖性抗增殖作用（10 IU/ml时P<0.01；1000 IU/ml时P<0.01和P<0.001）。单一IFN剂量的抗增殖作用最长持续4天，之后再次观察到黑色素瘤细胞不受抑制的增殖。在所有实验中，当使用浓度低100 - 1000倍时，天然干扰素β对所测试的两种黑色素瘤细胞系（StML - 11、SKMel - 28）显示出与重组干扰素α2a相似的抗增殖作用。在10000 IU/ml重组干扰素α2a和100 - 1000 IU/ml人干扰素β的浓度下观察到细胞毒性作用。
Zotero 插件